Identification and expression-profiling of Xenopus tropicalis miRNAs including plant miRNA-like RNAs at metamorphosis
Identification and expression-profiling of Xenopus tropicalis miRNAs including plant miRNA-like RNAs at metamorphosis
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DOI：
10.1016/j.febslet.2007.05.054
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发表时间：
2007-06-26
期刊：
影响因子：
3.5
通讯作者：
Kawahara, Akira
中科院分区：
文献类型：
--
作者：
Hikosaka, Akira;Takaya, Kenji;Kawahara, Akira
在热带爪蟾（Xenopus tropicalis）miRNA cDNA文库中发现了与某些植物miR 159相同的小RNA（miR 159-like RNA）（30个miRNA家族，由75个独特序列组成）。在发育过程中，神经组织中发现了这种RNA的优先表达。在21个可用的动物基因组数据库中没有发现与这种RNA种类匹配的序列，但在X.热带植物数据库。将讨论miR 159基因从植物中水平转移的可能性。通过DNA阵列和/或北方杂交显示其他miRNA种类在变态时的表达谱。(c)2007年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Small RNAs (miR159-like RNAs) identical to some plant miR159s were found in Xenopus tropicalis miRNA cDNA libraries (30 miRNA families consisting of 75 unique sequences). Preferential expression of this RNA species was found in neural tissues during development. A sequence matching to this RNA species was not found in the 21 available animal's genome databases, but its resembling sequences associated with transposons were found in the X. tropicalis database. A possibility of horizontal transfer of the miR159 genes from plants will be discussed. Expression profiles of other miRNA species at metamorphosis were shown by DNA array and/or Northern hybridization. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.