Formation of surface reaction products on bioactive glass and their effects on the expression of the osteoblastic phenotype and the deposition of mineralized extracellular matrix
Formation of surface reaction products on bioactive glass and their effects on the expression of the osteoblastic phenotype and the deposition of mineralized extracellular matrix
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DOI：
10.1016/s0142-9612(96)00059-2
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发表时间：
1997-02-01
期刊：
影响因子：
14
通讯作者：
Shapiro, IM
中科院分区：
文献类型：
--
作者：
ElGhannam, A;Ducheyne, P;Shapiro, IM
本研究的目的是检查碱离子释放、pH控制和缓冲能力对成骨细胞表型表达的影响。此外，我们确定了多孔生物活性玻璃（BG）的表面改性对体外培养的大鼠颅骨成骨细胞的活性的重要性。我们发现，在低的组织培养基（TCM）体积BG表面积（BG/SA）的比例，玻璃腐蚀的产品提高了TCM的pH值，对细胞活性产生不利影响，因此，由细胞合成的基质是非矿化的。另一方面，当pH/SA高且未超过培养基的缓冲能力时，细胞产生矿化的细胞外基质。解决第二个问题，我们观察到，BG表面的组成的修改显着影响成骨细胞的活性。仅用富磷酸钙层或血清蛋白层涂覆的BG改变了成骨细胞的表型特征。这些表面的存在降低了附着细胞的碱性磷酸酶活性;这一发现表明成骨细胞表型不保守。然而，当BG被涂覆有磷酸钙和血清蛋白的双层时，碱性磷酸酶（AP）活性升高，并且细胞外基质含有特征性骨标记物。我们的研究结果表明，富磷酸钙层促进吸附和浓缩的蛋白质从中药，它是由成骨细胞利用，形成矿化的细胞外基质。(C)1997年爱思唯尔科学有限公司。All rights reserved.
The objective of the study was to examine the effect of alkali ion release, pH control and buffer capacity on the expression of the osteoblastic phenotype. In addition, we determined the importance of modifications of the surface of porous bioactive glass (BG) on the activity of rat calvaria osteoblasts in vitro. We found that at a low tissue culture medium (TCM) volume to BG surface area (Vol/SA) ratio, the products of glass corrosion elevated the pH of the TCM to a value that adversely affected cellular activity; thus, the matrix synthesized by the cells was non-mineralized. On the other hand, when the Vol/SA was high and the buffer capacity of the medium was not exceeded, the cells generated a mineralized extracellular matrix. Addressing the second issue, we observed that modification of the composition of the BG surface markedly influenced osteoblast activity. BG that was coated with either a calcium phosphate-rich layer only or a serum protein layer changed the phenotypic characteristics of the osteoblasts. The presence of either of these surfaces lowered the alkaline phosphatase activity of the attached cells; this finding indicated that the osteoblast phenotype was not conserved. However, when the BG was coated with a bilayer of calcium phosphate and serum proteins, the alkaline phosphatase (AP) activity was elevated and the extracellular matrix contained characteristic bone markers. Our findings indicate that the calcium phosphate-rich layer promotes adsorption and concentration of proteins from the TCM, and it is utilized by the osteoblasts to form the mineralized extracellular matrix. (C) 1997 Elsevier Science Limited. All rights reserved.