Streptococcus gordonii pheromone s.g.cAM373 may influence the reservoir of antibiotic resistance determinants of Enterococcus faecalis origin in the oral metagenome.
Streptococcus gordonii pheromone s.g.cAM373 may influence the reservoir of antibiotic resistance determinants of Enterococcus faecalis origin in the oral metagenome.
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DOI：
10.1099/jmm.0.000613
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发表时间：
2017-10
影响因子：
3
通讯作者：
Jillian M. Mansfield;Paul Herrmann;A. Jesionowski;M. Vickerman
中科院分区：
文献类型：
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作者：
Jillian M. Mansfield;Paul Herrmann;A. Jesionowski;M. Vickerman
戈登链球菌产生一种信息素七肽 s.g.cAM373，它可在携带响应性质粒 pAM373 的粪肠球菌细胞中诱导接合交配反应。我们研究了这种属间信号传导对可能共存口腔生物膜的链球菌物种 DNA 获取的程度。将粪肠球菌/pAM373/pAMS470 细胞与合成的 s.g.cAM373、反向肽 s.g.cAM373-R 或无肽培养基一起孵育，并检查它们在 DNase 存在下将质粒 DNA 转移至链球菌物种的能力。用 s.g.cAM373 预诱导粪肠球菌供体，导致变形链球菌、血链球菌、咽峡炎链球菌和猪链球菌的非信息素产生菌株的转接合频率显着高于供体与 s.g.cAM373-R 或单独培养基预孵育时的频率。共生链球菌和携带信息素响应质粒的粪肠球菌之间肽介导的通讯可能促进接合DNA转移到旁观者物种，并影响口腔宏基因组中肠球菌起源的抗生素耐药性决定因素的储存。
Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. We investigated the extent of this intergeneric signaling on DNA acquisition by streptococcal species likely to cohabit oral biofilms. E. faecalis/pAM373/pAMS470 cells were incubated with synthetic s.g.cAM373, reverse peptide s.g.cAM373-R, or peptide-free medium and examined for their abilities to transfer plasmid DNA to streptococcal species in the presence of DNase. Preinduction of E. faecalis donors with s.g.cAM373 resulted in transconjugation frequencies in non-pheromone producing strains of Streptococcus mutans, Streptococcus sanguinis, Streptococcus anginosus, and Streptococcus suis that were significantly higher than frequencies when donors were preincubated with s.g.cAM373-R or medium alone. Peptide-mediated communication between commensal streptococci and E. faecalis carrying pheromone-responsive plasmids may facilitate conjugative DNA transfer to bystander species, and influence the reservoir of antibiotic resistance determinants of enterococcal origin in the oral metagenome.