Cardiac studies in rats with 11C-acetate and PET:: A comparison with 13N-ammonia
Cardiac studies in rats with 11C-acetate and PET:: A comparison with 13N-ammonia
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DOI：
10.1109/tns.2002.803679
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发表时间：
2002-10-01
影响因子：
1.8
通讯作者：
Lecomte, R
中科院分区：
文献类型：
--
作者：
Bentourkia, M;Croteau, É;Lecomte, R
局部心肌血流量 (MBF) 可以通过动态正电子发射断层扫描 (PET) 和 N-13-氨或 C-11-乙酸盐进行无创评估。后者还可用于从同一时间-活动曲线估计心肌耗氧量 (MVO2)。在这项工作中，我们探索了这种技术通过使用 Sherbrooke 小动物 PET 扫描仪进行动态成像来评估正常和缺血大鼠（类似于 350 g）心肌参数的可行性。以列表模式获取 N-13-氨和 11 C-乙酸盐的扫描，作为两次连续的 15 分钟动态扫描，间隔 5 分钟，无需将大鼠从扫描仪移开。在心肌的四个部分上定义感兴趣区域（ROI）以提取时间-活动曲线。应用三室模型分别用三个和四个速率常数以及血容量来拟合 N-13-氨和 C-11-乙酸数据。从 N-13-氨和 C-11-乙酸盐中提取的 MBF 估计值之间观察到了合理的一致性。由 k(2) 速率常数给出的 MVO2 估计值具有良好的重现性，表明可以使用 C-11-乙酸盐与 MBF 同时可靠地评估心肌代谢。结论是，通过N-13-氨和C-11-乙酸的高速、列表模式、动态成像和动力学模型，可以评估小动物PET扫描大鼠的心肌血流量和耗氧量。
Regional myocardial blood flow (MBF) can be assessed noninvasively with dynamic positron emission tomography (PET) and N-13-ammonia or C-11-acetate. The latter can also be used to estimate the myocardial oxygen consumption (MVO2) from the same time-activity curve. In this work, we explore the feasibility of such techniques for evaluating myocardial parameters in normal and ischemic rats (similar to350 g) by dynamic imaging with the Sherbrooke small animal PET scanner. Scans were acquired in list mode for N-13-ammonia and 11 C-acetate as two consecutive 15-min dynamic scans separated by a 5-min interval, without moving the rat from the scanner. Regions of interest (ROIs) were defined on four segments of the myocardium to extract the time-activity curves. Three-compartment models were applied to fit the N-13-ammonia and C-11-acetate data with three and four rate constants, respectively, plus blood volume. A reasonable agreement was observed between MBF estimates extracted from N-13-ammonia and C-11-acetate. MVO2 estimates, as given by the k(2) rate constant, were obtained with good reproducibility, indicating that myocardial metabolism can reliably be assessed concurrently with MBF using C-11-acetate. It is concluded that myocardial blood flow and oxygen consumption can be evaluated in rats with small animal PET scantiers through high-rate, list-mode, dynamic imaging and kinetic modeling of N-13-ammonia and C-11-acetate.