Transmembrane segments of syntaxin line the fusion pore of Ca2+-triggered exocytosis
Transmembrane segments of syntaxin line the fusion pore of Ca2+-triggered exocytosis
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DOI：
10.1126/science.1095801
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发表时间：
2004-04-09
期刊：
影响因子：
56.9
通讯作者：
Jackson, MB
中科院分区：
文献类型：
--
作者：
Han, X;Wang, CT;Jackson, MB
调节胞吐作用的融合孔是连接并跨越囊泡和质膜的通道。这种重要的胞吐作用中间结构的分子组成尚不清楚。在这里，我们发现突触蛋白（Syx）（一种胞吐作用所必需的质膜蛋白）跨膜片段内的一些残基的突变，改变了通过融合孔的神经递质通量并改变了孔电导。影响融合孔通量的残留物位于α螺旋模型的一个面上。因此，融合孔至少部分地由质膜中5至8个Syx跨膜片段的圆形排列形成。
The fusion pore of regulated exocytosis is a channel that connects and spans the vesicle and plasma membranes. The molecular composition of this important intermediate structure of exocytosis is unknown. Here, we found that mutations of some residues within the transmembrane segment of syntaxin (Syx), a plasma membrane protein essential for exocytosis, altered neurotransmitter flux through fusion pores and altered pore conductance. The residues that influenced fusion-pore flux lay along one face of an alpha-helical model. Thus, the fusion pore is formed at least in part by a circular arrangement of 5 to 8 Syx transmembrane segments in the plasma membrane.