Determination of flavonoids in Portulaca oleracea L. by capillary electrophoresis with electrochemical detection
Determination of flavonoids in Portulaca oleracea L. by capillary electrophoresis with electrochemical detection
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DOI：
10.1016/j.jpba.2006.01.013
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发表时间：
2006-05-03
影响因子：
3.4
通讯作者：
Chen, Guonan
中科院分区：
文献类型：
--
作者：
Xu, Xueqin;Yu, Lishuang;Chen, Guonan
建立了毛细管电泳-电化学检测法（CE-ED），用于植物中山奈酚、芹菜素、杨梅素、槲皮素和木犀草素5种黄酮类化合物的定性和定量分析。通过对运行缓冲液、pH和浓度、分离电压、进样时间、检测电位等因素的考察，确定了最佳的分离条件。工作电极是位于毛细管出口对面的直径为500 μ m的碳盘电极。在室温下，以50 mM Na_2B_4O_7 -100 mM NaH_2PO_4（pH 8.50）为运行缓冲液，分离电压19.5 kV，在60 cm长的毛细管柱上，5种黄酮类化合物在21 min内得到良好分离。峰电流和分析物浓度之间的关系是线性超过约两个数量级，和检测限（S/N = 3）的范围为0.12至0.21 μ g/ml的所有分析物。采用优化后的毛细管电泳法对马齿苋不同部位中上述黄酮类化合物进行了分析。(c)2006 Elsevier B. V.保留所有权利。
A general method based on capillary electrophoresis with electrochemical detection (CE-ED) was developed for identification and determination of five flavonoids (kaempferol, apigenin, myricetin, quercetin and luteolin) in plant species. Running buffer, pH and concentration, separation voltage, injection time and detection potential were investigated to acquire the optimum conditions. The working electrode was a 500 mu m diameter carbon disc electrode positioned opposite the outlet of capillary. At room temperature, the five flavonoids could be well separated within 21 min in a 60 cm length capillary at a separation voltage of 19.5 kV with 50 mM Na2B4O7-100 mM NaH2PO4 (pH 8.50) as the running buffer. The relationship between peak currents and analyte concentrations was linear over about two orders of magnitude, and the detection limits (S/N = 3) were ranging from 0.12 to 0.21 mu g/ml for all analytes. The optimized CE-ED method was employed to analyze the above flavonoids in different parts of Portulaca oleracea L. (c) 2006 Elsevier B.V. All rights reserved.