Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584
Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584
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DOI：
10.1128/aem.66.10.4325-4333.2000
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发表时间：
2000-10-01
影响因子：
4.4
通讯作者：
Hammes, WP
中科院分区：
文献类型：
--
作者：
Gänzle, MG;Höltzel, A;Hammes, WP
鲁氏乳杆菌LTH2584的抗菌活性既不能归因于细菌素，也不能归因于reurin或有机酸的产生。我们纯化了活性化合物，命名为reurcyclin，并对其抗菌活性进行了表征。环素对乳杆菌、枯草芽孢杆菌、蜡样芽孢杆菌、粪肠球菌、金黄色葡萄球菌、无毒李斯特菌等具有广谱抑菌作用，对革兰氏阴性菌生长无影响，但对大肠杆菌脂多糖突变株生长有抑制作用。雷公藤环素对桑氏乳杆菌、金黄色葡萄球菌和枯草杆菌有明显的杀菌作用，对L、桑氏杆菌的裂解作用呈剂量依赖性。枯草杆菌孢子的萌发受到抑制，但在不允许萌发的条件下，孢子不受影响。培养基中的脂肪酸供应对Reurcyclin的产量及其在生产细胞和培养上清液中的分布有很大的影响。通过溶剂提取、凝胶过滤、RP-C-8柱层析和阴离子交换层析，从L、Reuri LTH2584的细胞提取物和培养上清液中纯化了Reuryclin，然后用反相高压液相色谱重层析，Reurcyclin被鉴定为一个带负电的高度疏水的分子，分子质量为349 Da，结构鉴定为(A，Holtzel，M，G，Ganzle，G，J，Nicholson，W.P，Hammes，和G，Jung，Angw，Chem，Int)。艾德39：2766-2768,2000)揭示了reurcyclin是一种新的四酸衍生物。L、reuri LTH2584培养上清液的抑制活性与纯化的和合成的reuryclin的抑制活性相当。
Lactobacillus reuteri LTH2584 exhibits antimicrobial activity that can be attributed neither to bacteriocins nor to the production of reuterin or organic acids. We have purified the active compound, named reutericyclin, to homogeneity and characterized its antimicrobial activity. Reutericyclin exhibited a broad inhibitory spectrum including Lactobacillus spp,, Bacillus subtilis, B. cereus, Enterococcus faecalis, Staphylococcus aureus, and Listeria innocua, It did not affect the growth of gram-negative bacteria; however, the growth of lipopolysaccharide mutant strains of Escherichia coli was inhibited. Reutericyclin exhibited a bactericidal mode of action against Lactobacillus sanfranciscensis, Staphylococcus aureus, and B. subtilis and triggered the lysis of cells of L, sanfranciscensis in a dose-dependent manner. Germination of spores of B. subtilis was inhibited, but the spores remained unaffected under conditions that do not permit germination. The fatty acid supply of the growth media had a strong effect on reutericyclin production and its distribution between producer cells and the culture supernatant, Reutericyclin was purified from cell extracts and culture supernatant of L, reuteri LTH2584 cultures grown in mMRS by solvent extraction, gel filtration, RP-C-8 chromatography, and anion-exchange chromatography, followed by rechromatography by reversed-phase high-pressure liquid chromatography, Reutericyclin was characterized as a negatively charged, highly hydrophobic molecule with a molecular mass of 349 Da, Structural characterization (A, Holtzel, M, G, Ganzle, G, J, Nicholson, W. P, Hammes, and G, Jung, Angew, Chem, Int. Ed. 39:2766-2768, 2000) revealed that reutericyclin is a novel tetramic acid derivative. The inhibitory activity of culture supernatant of L, reuteri LTH2584 corresponded to that of purified as well as synthetic reutericyclin.