Ciz1 promotes tumorigenicity of prostate carcinoma cell
Ciz1 promotes tumorigenicity of prostate carcinoma cell
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Ciz1 促进前列腺癌细胞的致瘤性。
DOI：
10.2741/4331
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Kong, Chuize
中科院分区：
文献类型：
--
作者：
Liu, Tao;Ren, Xiaohui;Kong, Chuize
前列腺癌是男性最常见的恶性肿瘤，也是发达国家癌症相关死亡的第二大原因。最近的工作揭示了 CIP 相互作用锌指蛋白 1 (CIZ1) 在癌细胞生物学中的重要性，但其在前列腺癌中的作用尚不清楚。我们的研究比较了储存的前列腺癌与匹配的副肿瘤组织以及不同来源的肿瘤细胞系中的 CIZ1 基因表达。这项研究表明，CIZ1在高级别前列腺癌中的表达高于低级别前列腺癌和正常组织。在肿瘤细胞系中，PC-3表现出最高水平的CIZ1表达。 PC-3细胞中的CIZ1基因沉默减少了细胞增殖和集落形成，诱导G1细胞周期停滞，抑制裸鼠肿瘤形成，并抑制前列腺癌相关基因的表达。这些结果表明CIZ1可能在人类前列腺癌的进展中发挥重要作用，并且可以作为前列腺癌的治疗靶点。
Prostate cancer is the most common malignancy in men and is the second leading cause of cancer-related mortality in developed countries. Recent work has revealed the significance of CIPinteracting zinc finger protein 1 (CIZ1) in cancer cell biology, but its roles in prostatic carcinoma are unknown. Our study compared CIZ1 gene expression in banked prostatic carcinomas versus matched paraneoplastic tissues and in tumor cell lines of varying origin. This study revealed that the expression of CIZ1 was higher in high-grade prostate cancer than in low-grade prostate cancer and normal tissues. Among the tumor cell lines, PC-3 exhibited the highest levels of CIZ1 expression. CIZ1 gene silencing in PC-3 cells reduced cell proliferation and colony formation, induced cell cycle arrest in G1, inhibited tumor formation in nude mice, and suppressed the expression of genes related to prostate carcinoma. These results suggest that CIZ1 may play an important role in the progression of human prostate carcinoma and us which may be used as a therapeutic target in prostate cancer.