Fifty-four new gene-based canine microsatellite markers.
Fifty-four new gene-based canine microsatellite markers.
复制标题
五十四个新的基于基因的犬微卫星标记。
DOI：
10.1093/jhered/esi090
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Jakobs,PM
中科院分区：
文献类型：
--
作者：
Litt,M;Bestwick,ML;Winther,MJ;Jakobs,PM
开发了54个新的标记，以填补目前犬类微卫星图谱中的空白，并补充在高度近交系犬谱系中可能信息不足的现有标记。利用辐射杂交图谱中包含的犬类基因获得人类同源基因序列。利用BLAST检索与犬全基因组猎枪（canine whole genome shotgun, wgs）序列资源比对，获得了犬基因组序列中与人类基因同源的序列。利用包含微卫星的犬类序列，并将其映射回人类基因组的正确位置，设计引物从犬类基因组DNA中扩增微卫星。标记的杂合性通过从雀巢普瑞纳参考家族DNA分布中心获得的祖代DNA以及无亲缘关系的布维耶犬和爱尔兰狼犬的DNA进行基因分型检测。在2004年7月冻结的犬基因组组装中，用PCR或BLAST方法确定了标记的犬图谱位置。
Fifty-four new markers were developed to fill in gaps in the current map of canine microsatellites and to complement existing markers that may not be sufficiently informative in highly inbred canine pedigrees. Canine genes contained on the radiation hybrid map were used to obtain the sequence of the human homolog. A BLAST search versus the canine whole genome shotgun (wgs) sequence resource was used to obtain the sequence of the canine genomic contigs containing the homolog of the corresponding human gene. Canine sequences that contained microsatellites and mapped back to the correct location in the human genome were used to design primers for amplification of the microsatellites from canine genomic DNA. Heterozygosities of the markers were tested by genotyping grandparental DNAs obtained from the Nestle Purina Reference family DNA distribution center plus DNAs from unrelated Bouviers and Irish wolfhounds. Canine map positions of markers on the July 2004 freeze of the canine genome assembly were determined by in silico PCR or BLAST.