Overexpression ofCcNAC1gene promotes early flowering and enhances drought tolerance of jute (Corchorus capsularis L.)
Overexpression ofCcNAC1gene promotes early flowering and enhances drought tolerance of jute (Corchorus capsularis L.)
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CcNAC1基因的过表达促进黄麻（Corchorus capsularis L.）提早开花并增强耐旱性
DOI：
10.1007/s00709-020-01569-y
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发表时间：
2020-10-20
期刊：
影响因子：
2.9
通讯作者：
Qi，Jianmin
中科院分区：
文献类型：
--
作者：
Zhang，Gaoyang;Huang，Siqi;Qi，Jianmin
干旱是严重影响植物生长的主要因素，对黄麻作物生产具有破坏性影响。NAC(NAM、ATAF和CUC2)转录因子是植物中的一个大基因家族，已被证明在调节植物发育过程和非生物胁迫抗性方面发挥着重要作用。本研究克隆了NAC转录因子CcNAC1，并对其在黄麻中的功能进行了研究。RT-qPCR分析表明，CcNAC1的表达在干旱胁迫8h后达到高峰。通过农杆菌介导的遗传转化，获得了CcNAC1过表达和基因敲除植株。PCR和Southern杂交结果表明，CcNAC1基因已整合到黄麻基因组中。与对照相比，CcNAC1基因的过表达加速了植株的生长，促进了植株的早花，提高了植株的抗旱性。3-酮酰辅酶A合成酶(KCS)基因在CcNAC1过表达植株中的表达水平显著升高，而在基因敲除植株中的表达水平显著降低，表明CcNAC1转录因子调控KCS基因的表达。酵母-2-杂交(Y2H)实验证实了CcNAC1和KCS之间的物理相互作用。这些结果为CcNAC1基因调控植物生长和抗旱性的分子机制提供了较为全面的信息，表明CcNAC1基因在黄麻抗旱性中起着积极的调节作用。
Drought is the main factor that significantly affects plant growth and has devastating effects on crop production of jute. NAC (NAM, ATAF, and CUC2) transcription factors (TFs) are a large gene family in plants that have been shown to play many important roles in regulating developmental processes and abiotic stress resistance. In this study, a NAC transcription factor, CcNAC1, was cloned and characterized its function in jute. RT-qPCR analysis showed that CcNAC1 expression peaks after 8 h of drought stress. CcNAC1 overexpression and knockdown plants were created by Agrobacterium-mediated genetic transformation. PCR and southern hybridization results indicate that theCcNAC1gene was integrated into the genome of jute. Overexpression of theCcNAC1gene sped up the plant growth, promoted early flowering, and increased drought tolerance compared to the control plants. 3-Ketoacyl-CoA synthase (KCS) gene expression level increased significantly in the CcNAC1-overexpression plants and decreased in knockdown plants, which showed that CcNAC1 transcription factor regulated KCS gene expression. Yeast-2-Hybrid (Y2H) assays validated the physical interaction between CcNAC1 and KCS. The results provide relatively comprehensive information on the molecular mechanisms ofCcNAC1gene underlying the regulation of plant growth and drought stress resistance, and indicate that CcNAC1 acts as a positive regulator in drought tolerance in jute (Corchorus capsularis L.).