A novel regulatory protein governing biofilm formation in Bacillus subtilis
A novel regulatory protein governing biofilm formation in Bacillus subtilis
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DOI：
10.1111/j.1365-2958.2008.06201.x
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发表时间：
2008-06-01
影响因子：
3.6
通讯作者：
Losick, Richard
中科院分区：
文献类型：
--
作者：
Chu, Frances;Kearns, Daniel B.;Losick, Richard
细胞外基质的产生是生物膜形成的标志。在芽孢形成细菌枯草芽孢杆菌中，基质由由epsA-O操纵子指定的胞外多糖和由yqxM-sipW-tasA操纵子编码的分泌蛋白TasA组成。过去和现在的证据表明epsA-O和yqxM-sipW-tasA操纵子由阻遏蛋白SinR和AbrB控制。在这里，我们报告了一种新的调节蛋白Slr的识别，促进转录的yqxM-sipW-tasA操纵子，但不需要的epsA-O操纵子的表达。我们进一步表明，该基因的Slr本身是负控制的SinR和AbrB。这些发现表明，基质生产是由一个复杂的网络，涉及负和正作用的调节蛋白的相互作用。
Production of an extracellular matrix is a hallmark of biofilm formation. In the spore-forming bacterium Bacillus subtilis, the matrix consists of an exopolysaccharide, which is specified by the epsA-O operon, and a secreted protein TasA, which is encoded by the yqxM-sipW-tasA operon. Past and present evidence establish that the epsA-O and yqxM-sipW-tasA operons are controlled by the repressor proteins SinR and AbrB. Here, we report the identification of a novel regulatory protein Slr that promotes transcription of the yqxM-sipW-tasA operon but is not needed for expression of the epsA-O operon. We further show that the gene for Slr is itself under the negative control of SinR and AbrB. These findings reveal that matrix production is governed by an intricate network involving the interplay of negatively and positively acting regulatory proteins.