Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue
Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue
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DOI：
10.1007/978-1-60761-746-4_12
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Yost, Richard A.
中科院分区：
文献类型：
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作者：
Garrett, Timothy J.;Yost, Richard A.
我们描述了中压MALDI和串联质谱（MS/MS和MSn）的实用程序的表征和脑组织切片中的磷脂成像。使用MS/MS光谱和MS/MS图像可以识别同量异位素化合物。磷脂酰胆碱，磷脂酰丝氨酸，磷脂酰乙醇胺和鞘磷脂直接从组织切片的结构表征。
We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MSn) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structural characterization of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and sphingomyelins directly from tissue sections is described.