Photochemical Protein Fixation on Polymer Surfaces via Derivatized Phenyl Azido Group
Photochemical Protein Fixation on Polymer Surfaces via Derivatized Phenyl Azido Group
复制标题
通过衍生的苯基叠氮基团将光化学蛋白质固定在聚合物表面
DOI：
10.1021/la00006a067
复制
发表时间：
1995
期刊：
影响因子：
3.9
通讯作者：
T. Sugawara
中科院分区：
文献类型：
--
作者：
T. Matsuda;T. Sugawara
本文提出了一种新型表面处理技术，旨在以微米级精度将蛋白质化学固定在基材表面上，该技术基于衍生于聚合物表面上蛋白质的苯基叠氮基团的光化学反应性。制备了苯基叠氮衍生的蛋白质（白蛋白和明胶）和合成聚合物（聚（3-叠氮苯乙烯）和聚（N,N-二甲基丙烯酰胺-共-3-叠氮苯乙烯）（IV））。将这些光反应性蛋白质从它们各自的水溶液吸附到聚合物表面上或将未修饰的蛋白质吸附到聚(3-叠氮苯乙烯)预涂表面上，并进行随后的紫外线照射。蛋白质的化学固定通过表面元素分析、电子光谱化学分析和使用相应抗体的比色染色来验证。原子力显微镜观察表明，这种光诱导蛋白质固定方法使我们能够在给定区域以微米级精度通过光化学方式将蛋白质固定在聚合物表面上。
This paper addresses a novel surface processing technology aimed at chemical fixation of proteins on substrate surfaces with micron-order precision that is based on the photochemical reactivity of a phenyl azido group derivatized either to a protein on a polymer surface. Phenyl azido-derivatized proteins (albumin and gelatin) and synthetic polymers (poly(3-azidostyrene) and poly(N,N-dimethylacrylamide-co-3-azidostyrene) (IV)) were prepared. The adsorption of these photoreactive proteins onto polymer surfaces or nonmodified protein onto a poly(3-azidostyrene)-precoated surface from their respective aqueous solutions and subsequent UV irradiation were performed. The chemical fixation of proteins was verified with surface elemental analysis by electron spectroscopy for chemical analysis and by colorimetric staining using corresponding antibodies. Atomic force microscopic observations showed that this photoinduced protein fixation method allows us to photochemically immobilize a protein on a polymer surface with micron-order precision in a given region.