Sensitivity and specificity of Congo red staining according to Romhanyi.: Comparison with Puchtler's or Bennhold's methods
Sensitivity and specificity of Congo red staining according to Romhanyi.: Comparison with Puchtler's or Bennhold's methods
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DOI：
10.1016/j.acthis.2006.03.017
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发表时间：
2006-01-01
影响因子：
2.5
通讯作者：
Makovitzky, Josef
中科院分区：
文献类型：
--
作者：
Bely, Miklos;Makovitzky, Josef
各种刚果红染色方法的敏感性和特异性在淀粉样变性的诊断中非常重要。当使用不太敏感的染色方法时，一些淀粉样变性的真阳性病例仍然未被检测到。一种更高特异性的方法可能检测到更多的病例，并在沉积的早期阶段揭示淀粉样变性。本文讨论了Romhanyi的刚果红染色法，并与Puchtler和Bennhold的方法进行了比较。使用Romhanyi的技术，不存在酒精分化，因此没有染料分子从淀粉样蛋白丝上被洗掉。定向染料分子的结合对于偏振显微镜是最佳的。该方法使用极性亲水封固剂阿拉伯树胶。在这种含碳水化合物的亲水性介质中，刚果红分子平行于淀粉样蛋白丝的表面取向，符号为线性正，对应于局部光学染色反应的加性特征。否则，刚果红分子垂直于胶原蛋白表面取向，降低双折射强度，甚至引起胶原蛋白双折射原始符号的反转。随着酒精的分化，刚果红染料分子被提取，这降低了淀粉样蛋白沉积物的双折射，即最小。淀粉样蛋白沉积物可能被遗漏。使用非极性疏水封固剂加拿大香脂，胶原纤维和淀粉样蛋白表面上的刚果红染料分子将发生轴平行排列，导致具有绿色偏振颜色的附加拓扑光学反应和淀粉样变性的假阳性诊断（“幻影淀粉样变性”）。(C)2006 Etsevier GmbH. All rights reserved.
The sensitivity and specificity of various Congo red staining methods is very important in the diagnosis of amyloidosis. When using a less sensitive staining method, some true positive cases of amyloidosis remain undetected. A more highly specific method potentially detects more cases and reveals amyloidosis in an earlier stage of deposition. In this paper, the Congo red staining method according to Romhanyi is discussed in comparison with Puchtler's and Bennhold's methods. Using Romhanyi's technique, there is no alcoholic differentiation, and thus no dye molecules are washed off the amyloid filaments. The binding of the oriented dye molecules is optimal for polarization microscopy. With this method, the polar hydrophilic mounting medium, gum Arabic is used. Mounted in this carbohydrate-containing, hydrophilic medium, the Congo red molecules are oriented parallel to the surface of the amyloid filaments and the sign is linear positive, corresponding to an additive character of topo-optical staining reactions. Otherwise, the Congo red molecules are oriented perpendicular to the surface of collagen, reducing the intensity of birefringence and even inducing an inversion of the original sign of the collagen birefringence. With alcoholic differentiation, Congo red dye molecules are extracted and this decreases the birefringence of amyloid deposits, i.e. minimal. amyloid deposits may be missed. Using the apolar hydrophobic mounting medium, Canada balsam, an axis-parallel arrangement of Congo red dye molecules on the surface of collagen fibers and amyloid will occur, resulting in an additive topo-optical reaction with a green polarization color and a false positive diagnosis of amyloidosis ("phantom amyloidosis"). (C) 2006 Etsevier GmbH. All rights reserved.