Characterization of cold-induced remodelling reveals depot-specific differences across and within brown and white adipose tissues in mice
Characterization of cold-induced remodelling reveals depot-specific differences across and within brown and white adipose tissues in mice
复制标题
冷诱导重塑的表征揭示了小鼠棕色和白色脂肪组织之间和内部的储库特异性差异
DOI：
10.1111/apha.12688
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发表时间：
2016-08-01
影响因子：
6.3
通讯作者：
Yan, J. -Q.
中科院分区：
文献类型：
--
作者：
Jia, R.;Luo, X. -Q.;Yan, J. -Q.
AimBrown和米色脂肪组织通过线粒体解偶联蛋白1以热量的形式消耗能量，防止体温过低和潜在的肥胖。后者重新激发了人们对了解褐化过程的兴趣，以实现潜在的治疗益处。目的研究冷致小鼠棕色和白色脂肪组织变化和褐变的时间分布。方法将雄性C57BL/6J小鼠单独饲养于4℃冷暴露1、2、3、4、5和7d。每天测量食物摄入量和体重。结果冷暴露后大鼠摄食量增加，体重和脂肪细胞体积一过性减小。发现iBAT质量增加，而SWAT和eWAT发现一过性降低。形态分析、遗传分析(UCP-1、PGC-1和Elov13)和生化分析(UCP-1、PPAR和aP2)相结合的结果证实了仓库对冷暴露的反应。结论我们的结果证实了不同的BAT和WAT仓库对冷诱导变化的不同反应，并支持短期冷暴露的影响是通过iBAT的扩张、激活和增加产热能力以及SWAT和eWAT的褐变来实现的。
AimBrown and beige adipose tissues dissipate energy in the form of heat via mitochondrial uncoupling protein 1, defending against hypothermia and potentially obesity. The latter has prompted renewed interest in understanding the processes involved in browning to realize the potential therapeutic benefits. To characterize the temporal profile of cold-induced changes and browning of brown and white adipose tissues in mice.MethodsMale C57BL/6J mice were singly housed in conventional cages under cold exposure (4 degrees C) for 1, 2, 3, 4, 5 and 7days. Food intake and body weight were measured daily. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous (sWAT) and epididymal white adipose tissue (eWAT) were harvested for histological, immunohistochemical, gene and protein expression analysis.ResultsUpon cold exposure, food intake increased, whilst body weight and adipocyte size were found to be transiently reduced. iBAT mass was found to be increased, whilst sWAT and eWAT were found to be transiently decreased. A combination of morphological, genetic (Ucp-1, Pgc-1 and Elov13) and biochemical (UCP-1, PPAR and aP2) analyses demonstrated the depot-specific remodelling in response to cold exposure.ConclusionOur results demonstrate the differential responses to cold-induced changes across discrete BAT and WAT depots and support the notion that the effects of short-term cold exposure are achieved by expansion, activation and increasing thermogenic capacity of iBAT, as well as browning of sWAT and, to a lesser extent, eWAT.