Expression and characterization of delayed rectifying K+ channels in anterior rat taste buds.
Expression and characterization of delayed rectifying K+ channels in anterior rat taste buds.
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DOI：
10.1152/ajpcell.00115.2005
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发表时间：
2005-10
期刊：
影响因子：
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通讯作者：
Lidong Liu;D. R. Hansen;Insook Kim;T. Gilbertson
中科院分区：
文献类型：
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作者：
Lidong Liu;D. R. Hansen;Insook Kim;T. Gilbertson
Delayed rectifying K+ (DRK) channels in taste cells have been implicated in the regulation of cell excitability and as potential targets for direct and indirect modulation by taste stimuli. In the present study, we have used patch-clamp recording to determine the biophysical properties and pharmacological sensitivity of DRK channels in isolated rat fungiform taste buds. Molecular biological assays at the taste bud and single-cell levels are consistent with the interpretation that taste cells express a variety of DRK channels, including members from each of the three major subfamilies: KCNA, KCNB, and KCNC. Real-time PCR assays were used to quantify expression of the nine DRK channel subtypes. While taste cells express a number of DRK channels, the electrophysiological and molecular biological assays indicate that the Shaker Kv1.5 channel (KCNA5) is the major functional DRK channel expressed in the anterior rat tongue.
