Nipkow confocal imaging from deep brain tissues.
Nipkow confocal imaging from deep brain tissues.
复制标题
深部脑组织的尼普科夫共聚焦成像。
DOI：
10.1142/s0219635211002658
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发表时间：
2011
影响因子：
1.8
通讯作者：
Y. Ikegaya
中科院分区：
文献类型：
--
作者：
Yuji Takahara;N. Matsuki;Y. Ikegaya
从脑组织成像的问题之一是光散射。因此，多光子激光扫描显微镜被广泛用于光学访问位于组织深处的荧光信号。在这里，我们报告说，Nipkow型旋转盘单光子共聚焦显微镜，它体现了高时间分辨率和缓慢的光漂白，也能够成像组织的深度高达150 μm。使用Nipkow-disk显微镜，我们进行了功能性多细胞钙成像CA 3神经元在toto完整的海马制剂和星形胶质细胞在体内新皮质层1。Nipkow圆盘显微镜的这种新的应用扩展了这种类型的显微镜的潜在用途，并将有助于我们了解自然的神经元微电路。
One of the problems in imaging from brain tissues is light-scattering. Thus, multiphoton laser scanning microscopy is widely used to optically access fluorescent signals located deeply in tissues. Here we report that Nipkow-type spinning-disk one-photon confocal microscopy, which embodies high temporal resolution and slow photobleaching, is also capable of imaging tissues to a depth of up to 150 μm. Using a Nipkow-disk microscope, we conducted functional multi-cell calcium imaging of CA3 neurons from in toto intact hippocampal preparations and astrocytes from in vivo neocortical layer 1. This novel application of Nipkow-disk microscopy expands the potential usefulness of this type of microscopy and will contribute to our understanding of natural neuronal microcircuitry.