Concentration-dependent effects of natural polyamines on peptide chain initiation and elongation in a cell-free system of protein synthesis
Concentration-dependent effects of natural polyamines on peptide chain initiation and elongation in a cell-free system of protein synthesis
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天然多胺对无细胞蛋白质合成系统中肽链起始和延伸的浓度依赖性影响
DOI：
10.1007/bf01261388
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发表时间：
1990
影响因子：
4.3
通讯作者：
J. G. Georgatsos
中科院分区：
文献类型：
--
作者：
T. Giannakouros;H. Nikolakaki;J. G. Georgatsos
亚毫摩尔浓度的亚精胺和精胺在无细胞系统中刺激氨基酸进入蛋白质的速度，由内源或外源mRNA(TMV，珠蛋白)指导。这些多胺的刺激作用在起始和成熟两个阶段都有，在TMV或珠蛋白mRNA的情况下更加明显，大约是无多胺系统的2.3倍。在精胺存在的情况下，多聚体的数量和多聚体相关的放射性增加了大约2倍。大分子多肽的合成是刺激性事件的特征。然而，高浓度的亚精胺和精胺会强烈抑制蛋白质中的氨基酸结合。抑制作用在多肽伸长阶段表现明显。在内源性信使核糖核酸的情况下，过量的多胺的加入会导致对氨基酸掺入的非均匀抑制。一个最有趣的发现是，随着多胺浓度的增加，MR值分别为63000、44000、15500和12500的四条谱带的强度增加或至少保持不变，而其他谱带的强度减弱，表明在多胺存在下蛋白质的差异翻译。
Spermidine and spermine at submillimolar concentrations stimulate the rate of incorporation of amino acid into protein in a cell-free system, directed either by endogenous or exogenous mRNA (TMV, globin). The stimulatory effects of these polyamines are exerted at both the stages of initiation and elogation and are more pronounced in the case of TMV or globin mRNA, amounting to approximately 2.3-fold stimulation over the polyamine-free system. The number of polysomes and the polysome-associated radioactivity increase approximately 2-fold in the presence of spermine. Synthesis of large polypeptides is a characteristic feature of the stimulatory event. However, elevated concentrations of spermidine and spermine strongly inhibit amino acid incorporation into protein. Inhibition is manifest at the stage of peptide elongation. In the case of endogenous mRNA the addition of an excess of polyamines results in a non uniform inhibition of amino acid incorporation. A most interesting finding is that, with increasing concentrations of polyamines, the intensity of four bands with Mr values of 63000, 44000, 15500 and 12500 respectively, increases or leastwise remains constant while others fade, indicading differential translation of proteins in the presence of polyamines.
大鼠肝脏蛋白质合成系统的表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eisenstein,RS;Harper,AE
通讯作者：
Harper,AE
多胺抑制鸟氨酸脱羧酶和 S-腺苷甲硫氨酸脱羧酶 mRNA 的翻译。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kameji,T;Pegg,AE
通讯作者：
Pegg,AE