2dx_merge:: Data management and merging for 2D crystal images
2dx_merge:: Data management and merging for 2D crystal images
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DOI：
10.1016/j.jsb.2007.09.011
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发表时间：
2007-12-01
影响因子：
3
通讯作者：
Stahlberg, Henning
中科院分区：
文献类型：
--
作者：
Gipson, Bryant;Zeng, Xiangyan;Stahlberg, Henning
膜蛋白的电子结晶学是通过透射电子显微镜的低剂量成像和计算机图像处理来确定膜重组和二维结晶的膜蛋白的结构。我们之前已经推出了软件系统2dx，用于用户友好的2D晶体图像处理。其中心组件2DX_IMAGE基于MRC程序套件，允许选择全自动处理一个2D晶体图像。我们在这里介绍了程序2DX_MERGE，它帮助用户管理2D晶体图像处理项目，并促进来自多个图像的数据的合并。合并后的数据集可以作为重新处理所有图像的参考，这通常会提高最终重建的分辨率。可以迭代地应用图像处理和合并，直到达到收敛。2Dx在GNU通用公共许可证下可在http://2dx.org.上获得(C)2007 Elsevier Inc.保留所有权利。
Electron crystallography of membrane proteins determines the structure of membrane-reconstituted and two-dimensionally (2D) crystallized membrane proteins by low-dose imaging with the transmission electron microscope, and computer image processing. We have previously presented the software system 2dx, for user-friendly image processing of 2D crystal images. Its central component 2dx_image is based on the MRC program suite, and allows the optionally fully automatic processing of one 2D crystal image. We present here the program 2dx_merge, which assists the user in the management of a 2D crystal image processing project, and facilitates the merging of the data from multiple images. The merged dataset can be used as a reference to re-process all images, which usually improves the resolution of the final reconstruction. Image processing and merging can be applied iteratively, until convergence is reached. 2dx is available under the GNU General Public License at http://2dx.org. (c) 2007 Elsevier Inc. All rights reserved.