Inhibition of DNA synthesis in proliferating human diploid fibroblasts by fusion with senescent cytoplasts.
Inhibition of DNA synthesis in proliferating human diploid fibroblasts by fusion with senescent cytoplasts.
复制标题
通过与衰老细胞质融合抑制增殖的人二倍体成纤维细胞的 DNA 合成。
DOI：
10.1016/0014-4827(83)90424-x
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发表时间：
1983
影响因子：
3.7
通讯作者：
James R. Smith
中科院分区：
文献类型：
--
作者：
C. K. Drescher;James R. Smith
从衰老的人二倍体成纤维细胞制备细胞质。将细胞质与年轻的人二倍体成纤维细胞融合，并分析融合产物中的 DNA 合成。与未融合的年轻细胞或与年轻细胞融合的年轻细胞质相比，衰老细胞质融合产物的 DNA 合成受到抑制 (⩾40%)。这些结果与之前的实验一致，之前的实验表明，衰老和年轻人类二倍体成纤维细胞融合的异核体两个细胞核中的 DNA 合成均被阻断。此外，这些结果支持衰老细胞合成特定物质的假设，该特定物质存在于衰老细胞的细胞质中，抑制DNA合成。
Cytoplasts were prepared from senescent human diploid fibroblasts. The cytoplasts were fused to young human diploid fibroblasts and DNA synthesis was analyzed in the fusion products. DNA synthesis was inhibited (⩾40%) in the senescent cytoplast fusion products when compared to unfused young cells or young cytoplasts fused with young cells. These results are consistent with previous experiments that have shown the blockage of DNA synthesis in both nuclei of heterokaryons from fusions of senescent and young human diploid fibroblast cells. Furthermore, these results support the postulate that senescent cells synthesize a specific substance(s), which is present in the cytoplasm of the senescent cell that inhibits DNA synthesis.