Construction of BAC library for the amphibian Xenopus tropicalis
Construction of BAC library for the amphibian Xenopus tropicalis
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DOI：
10.1266/ggs.79.49
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发表时间：
2004-02-01
影响因子：
1.1
通讯作者：
Shimizu, N
中科院分区：
文献类型：
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作者：
Ishii, Y;Asakawa, S;Shimizu, N
热带非洲爪哇已经成为非洲爪哇两栖动物的替代模式，因为它更适合于遗传学和遗传学研究。我们构建了一个由100,000多个热带爪哇精子克隆组成的基因组BAC文库。对具有代表性的BAC克隆进行脉冲场凝胶电泳法分析表明，插入DNA的平均大小为100kb，我们估计文库的覆盖范围是热带爪哇基因组的6倍，其碱基对为1.7×10(9)。为了评估BAC文库，我们试图分离包含原钙粘附素伽马(PcdhGamma)基因的BAC克隆，发现分离的BAC克隆组装成两个独立的重叠群。这一结果表明，在热带疟原虫基因组中至少存在两个PcdhGamma基因簇。
Xenopus tropicalis has become an alternative model to the amphibian Xenopus laevis because it is better suited for genetic and genonnic studies. We have constructed a genomic BAC library consisting of over 100,000 clones from sperm of Xenopus tropicalis. Analysis by pulsed field gel electrophoresis of representative BAC clones indicated the average size of insert DNA to be 100 kb, and we estimated the library covers 6 times the Xenopus tropicalis genome of 1.7 x 10(9) base pairs. To evaluate the BAC library, we attempted to isolate BAC clones which contain a protocadherin gamma(Pcdhgamma) gene and found that the isolated BAC clones are assembled as two separate contigs. This result suggests the presence of at least two clusters for the Pcdhgamma gene in the genome of X. tropicalis.