Complement consumption gonococcal peptidoglycan.
Complement consumption gonococcal peptidoglycan.
复制标题
补充消耗淋球菌肽聚糖。
DOI：
10.1128/iai.35.2.442-448.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
Rosenthal,RS
中科院分区：
文献类型：
--
作者：
Petersen,BH;Rosenthal,RS
从淋病奈瑟氏菌中提取纯化的肽聚糖(PG)，检测其对正常人血清补体的消耗能力。超声PG(S-PG)是一种由可溶片段(相对分子质量大于10(6))和完整(不可溶)PG组成的异质混合物，可降低四种人血清中完整溶血补体的水平。这项活动所需的S-PG的最低浓度约为每毫升血清500微克的S-PG。溶菌酶完全消化S-PG，产生小于10(4)分子量的PG片段，消除了补体消耗活性。S-PG介导的补体消耗导致单个补体成分C4和C3的耗竭。当C4缺乏的人血清或正常人血清用镁离子-乙二醇双(β-氨基乙醚)-N，N-四乙酸特异性地破坏经典的补体途径活性时，补体的消耗不发生。兔抗PG抗体的加入大大增加了淋球菌PG介导的补体消耗。综上所述，这些数据表明淋球菌PG介导的补体消耗通过经典的补体途径发生，依赖于抗PG抗体的存在，并且需要PG的糖苷连接聚合物。淋球菌PG介导的补体水平降低的程度在不同人血清中差异很大，这可能反映了不同数量的淋球菌PG天然抗体，或不同水平的人PG水解酶(S)能够将PG降解为非活性片段，或两者兼而有之。
Purified peptidoglycan (PG) obtained from Neisseria gonorrhoeae was tested for the ability to consume complement in normal human sera. Sonicated PG (S-PG), a heterogeneous mixture of soluble fragments (molecular weight, greater than 10(6)), as well as intact (insoluble) PG, reduced the level of whole hemolytic complement in a pool of four human sera. The minimal concentration of S-PG required for this activity was approximately 500 micrograms of S-PG per ml of serum. Complete lysozyme digestion of S-PG, yielding PG fragments of less than 10(4) molecular weight, eliminated complement-consuming activity. S-PG-mediated complement consumption resulted in depletion of the individual complement components C4 and C3. Consumption of complement did not occur when C4-deficient human serum or normal human sera treated with Mg2+-(ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid to specifically impair classical complement pathway activity were used. The addition of rabbit anti-PG antibody greatly enhanced gonococcal PG-mediated complement consumption. Together, the data suggested that gonococcal PG-mediated complement consumption occurred via the classical complement pathway, was dependent on the presence of anti-PG antibody, and required glycosidically linked polymers of PG. Individual human sera varied widely in the extent of gonococcal PG-mediated reduction of complement levels, presumably a reflection of either different amounts of natural antibody to gonococcal PG, different levels of human PG hydrolase(s) capable of degrading PG to inactive fragments, or both.