Non-enzymatic covalent protein labeling using a reactive tag
Non-enzymatic covalent protein labeling using a reactive tag
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DOI：
10.1021/ja074176d
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发表时间：
2007-12-26
影响因子：
15
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
--
作者：
Nonaka, Hiroshi;Tsukiji, Shinya;Hamachi, Itaru
在此我们描述了一种利用肽标签和合成分子探针的互补识别对进行蛋白质共价标记的新方法。通过标签位点与探针之间基于选择性分子识别的反应，实现了对融合标签蛋白质的快速且特异性的共价标记。这种方法的优势包括对融合标签蛋白质进行简便的功能修饰以及高标记特异性，这在各种条件下甚至在细胞内的标记实验中都得到了证明。
We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.