Hollow selenium nanoparticles from potato extract and investigation of its biological properties and developmental toxicity in zebrafish embryos
Hollow selenium nanoparticles from potato extract and investigation of its biological properties and developmental toxicity in zebrafish embryos
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DOI：
10.1049/iet-nbt.2018.5228
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发表时间：
2019-05-01
影响因子：
2.3
通讯作者：
Muthukumaran, Azhaguchamy
中科院分区：
文献类型：
--
作者：
Chandramohan, Subburaman;Sundar, Krishnan;Muthukumaran, Azhaguchamy
使用马铃薯淀粉作为还原剂和封端剂，轻松合成空心硒纳米颗粒（hSeNPs）。利用紫外-可见光谱（UV）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）和zeta电位分析仪对hSeNPs的形貌和结构特征进行了表征。通过UV证实了hSeNPs的光学特性。通过FTIR证实hSeNP悬浮液中各种官能团的存在。SEM结果表明，合成的hSeNPs均匀分布，形状为圆形，具有中空。发现hSeNP的平均尺寸为约115 nm。EDX分析也证实了样品中hSeNP的存在。用范围从10至50 μ g/ml的各种浓度的hSeNP处理斑马鱼胚胎。在较高浓度（30-50 μ g/ml）下观察到异常心跳、胚囊水肿、眼水肿和头部水肿。观察到hSeNPs的浓度依赖性抗氧化活性。hSeNPs对革兰氏阳性菌枯草芽孢杆菌和革兰氏阴性菌大肠杆菌具有良好的抗菌活性。本研究的结果表明，马铃薯提取物降低了hSeNPs的毒性，并且在不久的将来，较低浓度的hSeNPs可以用于各种生物医学应用。
A facile synthesis of hollow selenium nanoparticles (hSeNPs) was prepared using potato starch as a reducing and capping agent. The morphological and structural characters of the hSeNPs were characterised by ultraviolet-visible spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX) and zeta potential analyser. The optical characteristics of hSeNPs were confirmed by UV. The presence of various functional groups in the hSeNPs suspension was confirmed by FTIR. The SEM results suggested that the synthesised hSeNPs were uniformly distributed and circular in shape with a hollow. The average size of the hSeNPs was found to be around 115 nm. The EDX analysis also confirmed the presence of hSeNPs in the sample. The zebrafish embryos were treated with hSeNPs of various concentrations ranging from 10 to 50 mu g/ml. Abnormalities such as improper heartbeat, embryo sac oedema, ocular oedema and head oedema were noted at higher concentrations (30-50 mu g/ml). A concentration-dependent antioxidant activity of hSeNPs was observed. The hSeNPs showed good antibacterial activity against gram-positive Bacillus subtilis and gram-negative Escherichia coli. The results of this study indicate that potato extract reduces the toxicity of hSeNPs and lower concentrations of hSeNPs could be used for various biomedical applications in near future.