Male fertility and occurrence of 2n gametes in East African Highland bananas (Musa spp.)
Male fertility and occurrence of 2n gametes in East African Highland bananas (Musa spp.)
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DOI：
10.1007/s10681-007-9637-6
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发表时间：
2008-11
期刊：
影响因子：
1.9
通讯作者：
R. Ssebuliba;A. Tenkouano;M. Pillay
中科院分区：
文献类型：
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作者：
R. Ssebuliba;A. Tenkouano;M. Pillay
由基因型产生的花粉的数量和质量是在选择用于育种的父本时要考虑的重要属性。本研究的目的是确定花粉的数量和质量，在13个选定的东非高地香蕉（EAHB）的关系，六个二倍体常用的父本。花粉量以0 - 6的等级评分，同时使用乙酰胭脂红甘油凝胶测定花粉量沿轴沿着的变化。实际的雄性育性是用EAHB品种的花粉测定的，以M.acuminataspp. burmannicoides，'Calcutta 4'为雌性。在显微镜下借助刻度目镜测量了200个有活力的花粉粒的直径。平均花粉粒直径和花粉粒直径差异显著。二倍体的花粉萌发率高于EAHB。节数对EAHB（P ≤ 0.01）和‘皮桑栗林’（P ≤ 0.001）花粉粒数的影响极显著。‘皮桑里林’和EAHB品种约有100个节，而其他二倍体约有200个节。EAHB品种产生的花粉比大多数二倍体少。EAHB品种的实际雄性育性较低（1粒/束），而加尔各答4号（223.3粒/束）和皮桑里林（13.7粒/束）的实际雄性育性较低。虽然EAHB品种的2n花粉产量很低（0 - 7%），但在新的育种方法中使用它们的潜力是存在的。
The quantity and quality of pollen produced by a genotype are important attributes to be considered in the selection of male parents for breeding. The objective of this research was to determine the quantity and quality of pollen in 13 selected East African Highland bananas (EAHB) in relation to six diploids commonly used as male parents. Pollen quantity was scored on a scale of 0–6 while the variation of pollen stainability along the rachis was determined using acetocarmine glycerol jelly. Actual male fertility was determined by using pollen from the EAHB varieties onM. acuminataspp.burmannicoides, ‘Calcutta 4’, as the female. The diameter of 200 viable pollen grains was measured under a microscope with the aid of a graduated eyepiece. Significant differences were obtained for mean pollen stainability and pollen diameter. The pollen stainability for diploids was higher than that for the EAHB. Node numbers contributed significantly to variation of pollen stainability in EAHB (P≤ 0.01) and ‘Pisang lilin’ (P≤ 0.001). ‘Pisang lilin’ and the EAHB varieties had about 100 nodes as opposed to about 200 in other diploids. The EAHB varieties produced less pollen than most of the diploids. The actual male fertility of EAHB varieties was low (1 seed/bunch), compared to that of ‘Calcutta 4’ (223.3 seeds/bunch) and ‘Pisang lilin’ (13.7 seeds/bunch). Although the production of 2npollen in EAHB varieties was low (0–7%), the potential exists for using them in new breeding approaches.