Effects of compatibility of deproteinized antler cancellous bone with various bioactive factors on their osteogenic potential.
Effects of compatibility of deproteinized antler cancellous bone with various bioactive factors on their osteogenic potential.
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DOI：
10.1016/j.biomaterials.2013.08.024
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发表时间：
2013-12
期刊：
影响因子：
14
通讯作者：
Xuehui Zhang;Ming-ming Xu;L. Song;Yan Wei;Yuanhua Lin;Wentao Liu;B. Heng;Hui Peng;Ying Wang-Ying
中科院分区：
文献类型：
--
作者：
Xuehui Zhang;Ming-ming Xu;L. Song;Yan Wei;Yuanhua Lin;Wentao Liu;B. Heng;Hui Peng;Ying Wang-Ying
Combinations of calcium phosphate scaffolds and bioactive factors are promising niche-mimetic solutions for repairing large-sized bone defects. However, the importance of compatibility between scaffolds and bioactive factors on their osteogenic outcomes has been largely ignored. This study aimed to investigate the compatibility of calcinated antler cancellous bone (CACB) scaffolds with various bioactive factors including icariin (ICA), velvet antler polypeptides (VAP) or recombinant human bone morphogenetic protein-2 (rhBMP-2) as well as their combinational osteogenic potentialin vitroandin vivo. Scanning electron microscopy and fourier transform infrared spectroscopy confirmed the uniform distribution and chemical stability of the reagents on CABC.In vitrorelease profiles showed relative steady release of ICA from ICA/CACB, burst VAP release from VAP/CACB, and minimal rhBMP-2 release from rhBMP-2/CACB composites. When compared with VAP and rhBMP-2, incorporation of ICA within CACB resulted in most increased cell attachment, proliferation, alkaline phosphatase activity, osteogenic gene expression, and mineralization of rat bone marrow mesenchymal stem cells. In rabbit mandible critical-sized defects, the most extensive osteogenesis and neovascularization were observed in the ICA/CACB group. Differences between the VAP/CACB and rhBMP-2/CACB groups were not apparent. Interestingly, low pro-inflammatory (TNF-α, IL-6) and high anti-inflammatory (IL-10) mRNA levels were observed at scaffold implantation sites which were in close association with amount of new bone formation. These findings highlight that the compatibility between scaffolds and bioactive factors should been taken into account when considering the formula of optimized bone defect repair.
