Carcinogenesis in canine bistomal heterotopic tracheal grafts.
Carcinogenesis in canine bistomal heterotopic tracheal grafts.
复制标题
犬双口异位气管移植物的致癌作用。
DOI：
10.1016/s0003-4975(10)60296-2
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Matsumura,K
中科院分区：
文献类型：
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作者：
Paladugu,RR;Hammond,WG;Benfield,JR;Jensen,T;Pak,H;Matsumura,K
成功的犬肺癌模型需要在全身麻醉下反复暴露局灶性支气管致癌物。为了简化癌变过程中呼吸道黏膜的系列研究，我们进行了双口自体异位气管蒂移植。这些移植物可以很容易地返回到原来的原位位置，这已被证明是研究粘膜变化可逆性的一种方法。多环芳烃应用于黏膜，每周3次，21 ~ 22个月。然后将致癌物以缓释方式释放的硅橡胶聚合物植入物放置在移植物中4至6周。一系列细胞学和组织学检查显示移植物粘膜发生不典型鳞状皮化生。在完成致癌物暴露后17至18个月手术切除的7例移植物中有4例出现粘膜乳头状瘤病。异位双口气管移植为研究呼吸道上皮癌变提供了一种有效的方法，无需反复全身麻醉。
Successful canine lung cancer models have required repeated focal bronchial carcinogen exposure under general anesthesia. To simplify serial studies of the respiratory mucosa during carcinogenesis, bistomal autologous heterotopic tracheal pedicle grafts have been made. These grafts can readily be returned to the original orthotopic site, and this has been shown to be a method with which to study reversibility of mucosal changes. Polycyclic aromatic hydrocarbons were applied topically to the mucosa three times a week for 21 to 22 months in 21 grafts. Implants of Silastic polymer from which carcinogen was released in sustained-release fashion were then left in the grafts for 4 to 6 weeks. Serial cytological and histological examinations showed development of atypical squamous metaplasia in the graft mucosa. Mucosal papillomatosis was noted in 4 of 7 grafts surgically excised 17 to 18 months after completion of carcinogen exposure. The heterotopic bistomal tracheal graft provides a useful method for studying respiratory epithelial carcinogenesis without repeated general anesthesia.