Determination of estrogens in human urine by high-performance liquid chromatography/diode array detection with ultrasound-assisted cloud-point extraction.
Determination of estrogens in human urine by high-performance liquid chromatography/diode array detection with ultrasound-assisted cloud-point extraction.
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DOI：
10.1016/j.ab.2011.10.007
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发表时间：
2012-02
影响因子：
2.9
通讯作者：
Y. Zou;Yanhua Li;Hua Jin;Huinan Tang;D. Zou;Mousheng Liu;Yaling Yang
中科院分区：
文献类型：
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作者：
Y. Zou;Yanhua Li;Hua Jin;Huinan Tang;D. Zou;Mousheng Liu;Yaling Yang
建立了一种新的高效的分析方法，用于提取和浓缩人体尿液中的3种雌激素(17β-雌二醇（βE2）、雌酮（E1）和己烯雌酚（DES），然后进行高效液相色谱分析。它是基于用非离子表面活性剂（tergiitol TMN-6）诱导胶束组织介质提取目标雌激素。采用超声技术提高提取效率。考察了表面活性剂浓度、萃取温度、萃取时间、样品pH、离子强度、离心时间等参数对萃取目标分析物的影响。在最佳条件下，βE2、E1和DES的线性范围为5.0 ~ 1000ng/ml。各校正曲线相关系数均大于0.997。人尿中βE2、E1和DES的相对标准偏差（RSD, n=5）为2.36 ~ 5.27%，检出限（LOD）分别为0.1、0.2和0.1ng/ml。结果表明，该方法可用于人尿中βE2、E1和DES的分析。
A novel and efficient analytical methodology is proposed for extracting and preconcentrating three kinds of estrogens (17β-estradiol (βE2), estrone (E1), and diethylstilbestrol (DES)) in human urine prior to high-performance liquid chromatography (HPLC) analysis. It is based on the induction of micellar organized medium by using a nonionic surfactant (Tergitol TMN-6) to extract the target estrogens. Ultrasound was applied to enhance the extraction efficiency. Parameters affecting the extraction of target analytes including the concentration of surfactant, temperature, extraction time, sample pH, ionic strength, and centrifuging time were investigated. Under the optimum conditions, the linear range of βE2, E1, and DES was from 5.0 to 1000ng/ml. All correlation coefficients of the calibration curves were higher than 0.997. The relative standard deviations (RSD, n=5) were 2.36–5.27% and the limits of detection (LOD) were 0.1, 0.2, and 0.1ng/ml for βE2, E1, and DES in human urine, respectively. The results indicated that the method was successfully applied for analyzing βE2, E1, and DES in human urine.