Effect of UV radiation on the infrared spectra of collagen
Effect of UV radiation on the infrared spectra of collagen
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DOI：
10.1016/0141-3910(95)00159-x
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发表时间：
1996-01-01
影响因子：
5.9
通讯作者：
Sionkowska, A
中科院分区：
文献类型：
--
作者：
Kaminska, A;Sionkowska, A
研究了紫外辐射对鼠尾腱胶原红外光谱形状的影响。用计算机迭代程序将光谱分解成高斯分量。得到了分离组分的特征吸收峰。经紫外光照射后，胶原蛋白的红外酰胺带向低频移动。这表明该蛋白质发生了结构变化。在紫外光照射下，组分条带的吸光度变化表明与胶原结合的水损失，主链降解，酰胺，CH 2-N，= CH 2和C=O键断裂，侧基如COOH，C=O，COO-，红外光谱分析可提供胶原分子光化学转化的一些信息，但其转化效率较低。
The influence of UV radiation on the shape of infrared spectra of collagen from a rat tail tendon was studied. The spectra were resolved into Gaussian components by means af an iterative computer program. Characteristic absorption peaks for the resolved components were obtained. After UV irradiation the infrared amide bands of collagen were shifted to lower frequencies. This indicates that structural changes in this protein take place.Changes of absorbance of the components bands, under UV irradiation, point to the loss of water bonded to collagen, degradation of main chains with scission of the amide, CH2-N, =CH2 and C=O bonds, abstraction of the side groups as COOH, C=O, COO-, and change of the ratio of the helical to the coil conformation of collagen molecules.IR analysis may provide some information about the photochemical transformations in collagen, although they occur with rather low efficiency.