Inositol trisphosphate production and amylase secretion in mouse pancreatic acini.
Inositol trisphosphate production and amylase secretion in mouse pancreatic acini.
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DOI：
10.1016/0006-291x(85)91800-5
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发表时间：
1985-08
影响因子：
3.1
通讯作者：
R. Powers;A. Saluja;M. Houlihan;M. Steer
中科院分区：
文献类型：
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作者：
R. Powers;A. Saluja;M. Houlihan;M. Steer
用（~ 3 H）-肌醇预标记的分散的小鼠胰腺腺泡对胆碱能和胆囊收缩素类似物产生反应，产生（~ 3 H）-三磷酸肌醇（~ 3 H-IP_3）、（~ 3 H）-IP_2和（~ 3 H）-IP_1。（3 H）-IP 3的产生非常迅速，在激素刺激后5秒内达到最大值。用10− 3 M卡巴胆碱刺激，（3 H）-IP 3增加到未刺激腺泡的13倍。这些结果表明，在小鼠胰腺腺泡的刺激-分泌偶联的机制可能进行的机制类似于许多其他系统，包括大鼠胰腺腺泡。该序列包括促分泌素刺激的磷脂酰肌醇周转和Ca 2+动员，即促分泌素刺激的IP 3的产生，其诱导细胞内Ca 2+的随后释放。这些观察结果不同于最近由Hokin-Neaverson和Sadeghian（J.Biol.Chem.259：1346 1984）报道的结果，后者在小鼠胰腺腺泡中未检测到激素刺激的IP 3生成。
Dispersed mouse pancreatic acini prelabelled with (3H)-myoinositol generated (3H)-inositol trisphosphate (3H-IP3), (3H)-IP2and (3H)-IP1in response to both cholinergic and cholecystokinin analogues. The generation of (3H)-IP3was very rapid, reaching a maximal value within 5 seconds following hormone stimulation. Stimulation with 10−3M carbachol increased (3H)-IP3to a value which was 13 times that found in unstimulated acini. These results indicate that the mechanism of stimulus-secretion coupling in mouse pancreatic acini may proceed by a mechanism similar to many other systems, including rat pancreatic acini. This sequence includes hormone-stimulated phosphatidylinositol turnover and Ca2+mobilization, i.e. secretagogue-stimulated generation of IP3which induces the subsequent release of intracellular Ca2+. These observations differ from those recently reported by Hokin-Neaverson and Sadeghian (J. Biol. Chem. 259: 1346 1984), in which no hormone stimulated IP3generation was detected in mouse pancreatic acini.