The cellulose synthase 3 (CesA3) gene of oomycetes: structure, phylogeny and influence on sensitivity to carboxylic acid amide (CAA) fungicides
The cellulose synthase 3 (CesA3) gene of oomycetes: structure, phylogeny and influence on sensitivity to carboxylic acid amide (CAA) fungicides
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DOI：
10.1016/j.funbio.2012.02.003
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发表时间：
2012-04-01
期刊：
影响因子：
2.5
通讯作者：
Gisi, Ulrich
中科院分区：
文献类型：
--
作者：
Blum, Mathias;Gamper, Hannes A.;Gisi, Ulrich
适当的病害防治对于减少卵菌引起的产量损失非常重要。今天，卵菌控制部分通过抗性育种来实现，但主要是通过应用单位点作用模式的杀真菌剂，包括羧酸酰胺（CAAs）。尽管大多数特定的目标，杀真菌活性可以不同，甚至在属于同一门的物种，但其潜在的机制往往知之甚少。在试图阐明系统发育的基础和潜在的分子机制的敏感性和耐受性CAAs，纤维素合成酶3（CesA3）基因的分离和表征，编码的目标网站这类杀菌剂。CesA3基因存在于本研究中包括的所有25个物种中，代表白蛋白目、纤甲目、霜霉目、腐霉目、Rhipidiales和水霉目，并且基于系统发育分析，能够很好地分辨所有不同的分类目。使用CAA杀真菌剂双炔酰菌胺（MPD）的敏感性试验表明，只有属于霜霉目的物种被杀真菌剂抑制。分子数据提供的证据表明，霜霉目和CAA耐受性物种之间观察到的对CAA敏感性的差异很可能是由CesA3中1109位的固有氨基酸构型引起的，可能影响杀真菌剂结合。本研究不仅成功地将各种卵菌的CAA敏感性与固有的CesA3靶位点构型联系起来，而且还将其与更广泛的系统发育背景联系起来。(C)2012年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
Proper disease control is very important to minimize yield losses caused by oomycetes in many crops. Today, oomycete control is partially achieved by breeding for resistance, but mainly by application of single-site mode of action fungicides including the carboxylic acid amides (CAAs). Despite having mostly specific targets, fungicidal activity can differ even in species belonging to the same phylum but the underlying mechanisms are often poorly understood.In an attempt to elucidate the phylogenetic basis and underlying molecular mechanism of sensitivity and tolerance to CAAs, the cellulose synthase 3 (CesA3) gene was isolated and characterized, encoding the target site of this fungicide class. The CesA3 gene was present in all 25 species included in this study representing the orders Albuginales, Leptomitales, Peronosporales, Pythiales, Rhipidiales and Saprolegniales, and based on phylogenetic analyses, enabled good resolution of all the different taxonomic orders. Sensitivity assays using the CAA fungicide mandipropamid (MPD) demonstrated that only species belonging to the Peronosporales were inhibited by the fungicide. Molecular data provided evidence, that the observed difference in sensitivity to CAAs between Peronosporales and CAA tolerant species is most likely caused by an inherent amino acid configuration at position 1109 in CesA3 possibly affecting fungicide binding. The present study not only succeeded in linking CAA sensitivity of various oomycetes to the inherent CesA3 target site configuration, but could also relate it to the broader phylogenetic context. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.