An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain.
An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain.
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Eph 相关受体蛋白酪氨酸激酶基因在发育中的小鼠后脑中分段表达。
作者：
P. Gilardi;M. Nieto;M. Frain;M. Mattei;A. Chestier;D. Wilkinson;P. Charnay
在寻找可能参与调节后脑分割的基因，我们已经分离出小鼠的cDNA克隆对应于假定的蛋白激酶基因的聚合酶链反应扩增的cDNA从9.5天的胚胎后脑。原位杂交分析表明，这些基因之一，Sek，表达在一个交替的片段限制模式在发展中的后脑。Sek cDNA的分离和分析覆盖整个编码序列表明，Sek编码一个假定的受体蛋白酪氨酸激酶，属于Eph家族。这些数据与Sek基因产物在参与后脑模式形成的信号转导过程中的作用一致。
In search of genes possibly involved in the regulation of hindbrain segmentation, we have isolated mouse cDNA clones corresponding to putative protein kinase genes by polymerase chain reaction amplification of cDNA from 9.5-day-old embryo hindbrains. In situ hybridization analysis revealed that one of these genes, Sek, was expressed in an alternating segment-restricted pattern in the developing hindbrain. Isolation and analysis of Sek cDNAs covering the entire coding sequence indicated that Sek encoded a putative receptor protein tyrosine kinase, belonging to the Eph family. These data are consistent with a role of the Sek gene product in a signal transduction process involved in pattern formation in the hindbrain.