Antimicrobial activity and safety evaluation of peptides isolated from the hemoglobin of chickens.
Antimicrobial activity and safety evaluation of peptides isolated from the hemoglobin of chickens.
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DOI：
10.1186/s12866-016-0904-3
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发表时间：
2016-12-05
期刊：
影响因子：
4.2
通讯作者：
Shi R
中科院分区：
文献类型：
--
作者：
Hu F;Wu Q;Song S;She R;Zhao Y;Yang Y;Zhang M;Du F;Soomro MH;Shi R
血红蛋白是生物肽的丰富来源。作为家禽屠宰设施的副产品甚至废水，鸡血是血红蛋白最丰富的来源之一。本研究通过琼脂扩散法、最小抑菌浓度(MIC)分析、最小杀菌浓度(MBC)分析、时间依赖性抑制和杀菌实验，对鸡血红蛋白抗菌肽(CHAP)进行了分离和抑菌、杀菌活性测定。结果表明，CHAP对19株细菌有较强的快速抑菌作用，其中包括9株多重耐药菌。通过细菌生物被膜和氯化钠通透性试验、透射电子显微镜和扫描电子显微镜对其抑菌机理进行了研究。此外，CHAP在不同温度和动物血浆中表现出较低的溶血活性、胚胎毒性和较高的稳定性。CHAP在动物医药、养殖业和环境保护等方面具有扩大生产和开发价值的巨大潜力。本文的在线版本(doi：10.1186/s12866-0160904-3)包含补充材料，授权用户可以使用。
Hemoglobin is a rich source of biological peptides. As a byproduct and even wastewater of poultry-slaughtering facilities, chicken blood is one of the most abundant source of hemoglobin. In this study, the chicken hemoglobin antimicrobial peptides (CHAP) were isolated and the antimicrobial and bactericidal activities were tested by the agarose diffusion assay, minimum inhibitory concentration (MIC) analysis, minimal bactericidal concentration (MBC) analysis, and time-dependent inhibitory and bactericidal assays. The results demonstrated that CHAP had potent and rapid antimicrobial activity against 19 bacterial strains, including 9 multidrug-resistant bacterial strains. Bacterial biofilm and NaCl permeability assays, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were further performed to detect the mechanism of its antimicrobial effect. Additionally, CHAP showed low hemolytic activity, embryo toxicity, and high stability in different temperatures and animal plasma. CHAP may have great potential for expanding production and development value in animal medication, the breeding industry and environment protection. The online version of this article (doi:10.1186/s12866-016-0904-3) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
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