Desynchronizing Embryonic Cell Division Waves Reveals the Robustness of Xenopus laevis Development
Desynchronizing Embryonic Cell Division Waves Reveals the Robustness of Xenopus laevis Development
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DOI：
10.1016/j.celrep.2017.09.017
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发表时间：
2017-10-03
期刊：
影响因子：
8.8
通讯作者：
Ferrell, James E., Jr.
中科院分区：
文献类型：
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作者：
Anderson, Graham A.;Gelens, Lendert;Ferrell, James E., Jr.
非洲爪哇早期胚胎充满了动态的空间波。其中一个这样的波，细胞分裂波，出现在整个胚胎中最初几十个和后来数百个细胞的集体细胞分裂时间。在这里，我们证明了细胞分裂波不在相邻细胞之间传播，也不依赖于细胞到细胞的耦合来维持它们的分裂定时。相反，分裂期间的内在变异自主地、逐渐地建立了这些显著的细胞分裂模式。通过将胚胎置于温度梯度中来破坏这种分裂模式，会导致高度不同步地进入中胚泡过渡，并导致中胚层标记Xbra的错误表达。值得注意的是，这种基因表达缺陷在退化过程中得到了纠正，导致了延迟但正常的Xbra表达和存活的胚胎。这意味着在退化过程中存在一种先前未知的机制来使中胚层基因表达正常化。
The early Xenopus laevis embryo is replete with dynamic spatial waves. One such wave, the cell division wave, emerges from the collective cell division timing of first tens and later hundreds of cells throughout the embryo. Here, we show that cell division waves do not propagate between neighboring cells and do not rely on cell-to-cell coupling to maintain their division timing. Instead, intrinsic variation in division period autonomously and gradually builds these striking patterns of cell division. Disrupting this pattern of division by placing embryos in a temperature gradient resulted in highly asynchronous entry to the midblastula transition and misexpression of the mesodermal marker Xbra. Remarkably, this gene expression defect is corrected during involution, resulting in delayed yet normal Xbra expression and viable embryos. This implies the existence of a previously unknown mechanism for normalizing mesodermal gene expression during involution.