Identification of yebG as a DNA damage-inducible Escherichia coli gene
Identification of yebG as a DNA damage-inducible Escherichia coli gene
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DOI：
10.1016/s0378-1097(97)00412-6
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发表时间：
1997-11-01
影响因子：
2.1
通讯作者：
de Almeida, DF
中科院分区：
文献类型：
--
作者：
Lomba, MR;Vasconcelos, AT;de Almeida, DF
大肠杆菌yebG基因上游的核苷酸序列与SOS系统调控位点（假定的SOS盒、-10和-35启动子盒以及核糖体结合位点）的特征相似。操纵子融合实验现在表明，该区域以recA和lexa依赖的方式控制转录，报告基因的表达可由丝裂霉素C处理后的DNA损伤诱导。表达的增加不是由于质粒拷贝数的增加。这些结果表明，yebG是一个新的SOS调控基因。预计yebC产物是一个96个氨基酸残基，10.7 kDa的蛋白质，其功能尚不清楚。与其他SOS基因不同，携带yebG调控区的构建体不是固定期诱导的。
The nucleotide sequence upstream of the Escherichia coli yebG gene presents features similar to those found in SOS system regulatory sites (putative SOS box, -10 and -35 promoter boxes and a ribosome binding site). Operon fusion assays demonstrate now that this region controls transcription in a recA-, lexA-dependent way and that the reporter gene expression is inducible by DNA damage consequent to mitomycin C treatment. Increased expression does not result from an increase in plasmid copy number. These results indicate that yebG is a novel SOS regulon gene. The yebC product is predicted to be a 96 amino acid residue, 10.7 kDa protein whose function is not yet known. Unlike other SOS genes, the construct carrying the yebG regulatory region is not stationary phase inducible.