Enhanced PCR amplification of GC-rich DNA templates by gold nanoparticles.
Enhanced PCR amplification of GC-rich DNA templates by gold nanoparticles.
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DOI：
10.1021/am4040448
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发表时间：
2013-11
影响因子：
9.5
通讯作者：
Wenchao Yang;Xinhui Li;Jielin Sun;Zhifeng Shao
中科院分区：
文献类型：
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作者：
Wenchao Yang;Xinhui Li;Jielin Sun;Zhifeng Shao
金纳米颗粒（AuNPs）已被报道，以促进双链DNA的解离和提高几个PCR系统的性能。在这里，我们研究了AuNPs对富含GC的DNA扩增的影响。我们发现，金纳米颗粒可以增强PCR扩增的GNAS 1启动子区（约84% GC）介导的Pfu或Taq DNA聚合酶。然而，在AuNP的最佳浓度下，需要更高量的Taq。此外，还能高效扩增出富含GC的智人FMR 1基因（GC率为80.4%）和铜绿假单胞菌exoT基因（GC率为67.3%）、exsE基因（GC率为71%）和pqqF基因（GC率为74%）。AuNPs可以成为富GC PCR的有效添加剂，并有助于基础和临床研究中具有挑战性的基因组序列的分析。
Gold nanoparticles (AuNPs) have been reported to facilitate double-stranded DNA dissociation and improve performance of several PCR systems. Here we investigated AuNPs' effect on GC-rich DNA amplification. We found that AuNPs could enhance PCR amplification of the GNAS1 promoter region (∼84% GC) mediated by Pfu or Taq DNA polymerase. However, under optimal concentrations of AuNPs, higher amounts of Taq were required. Furthermore, the GC-rich FMR1 (80.4% GC) gene of Homo sapiens as well as exoT (67.3% GC), exsE (71% GC) and pqqF genes (74% GC) of Pseudomonas aeruginosa were also efficiently amplified. AuNPs can become an effective additive in GC-rich PCR and facilitate analysis of challenging genomic sequence in basic and clinical research.