Deletion mapping of a short polyoma virus middle T antigen segment important for transformation
Deletion mapping of a short polyoma virus middle T antigen segment important for transformation
复制标题
对转化很重要的短多瘤病毒中间 T 抗原片段的缺失图谱
DOI：
10.1128/jvi.46.1.284-287.1983
复制
发表时间：
1983
影响因子：
5.4
通讯作者：
G. Magnusson
中科院分区：
文献类型：
--
作者：
S. Nilsson;C. Tyndall;G. Magnusson
Viable polyoma virus mutants were constructed that had small deletions in the early region of the genome. The deletions together removed most of the segment missing from the genome of the nontransforming mutant dl23 (N. Smolar and B. E. Griffin, J. Virol. 38:958-967, 1981). The transformation properties, as measured by colony formation in soft agar, of mutants with overlapping or contiguous deletions showed that part or all of the middle T antigen segment, consisting of the short amino acid sequence Glu4-Tyr-Met-Pro-Met, was essential for the activity of the protein in transformation. However, the segment could be deleted without significant effect on the in vitro protein kinase activity associated with the middle T antigen.
