Estimating age and growth in long-lived temperate freshwater crayfish using lipofuscin
Estimating age and growth in long-lived temperate freshwater crayfish using lipofuscin
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DOI：
10.1046/j.1365-2427.1998.00292.x
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发表时间：
1998-05-01
影响因子：
2.7
通讯作者：
Shelton, PMJ
中科院分区：
文献类型：
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作者：
Belchier, M;Edsman, L;Shelton, PMJ
1.在淡水螯虾的生态学研究中，由于缺乏合适的年龄估计方法，导致种群基本参数的确定较为复杂.以前，脂褐质年龄色素在短寿命的热带小龙虾的嗅叶细胞团（OLCM）已被用于准确的年龄测定。在这里，我们提出了第一次测试这种方法的寿命较长，温带物种，信号小龙虾，小龙虾。共聚焦荧光显微镜和图像分析的组织切片被用来量化OLCM脂褐素在瑞典P，leniusculus从几个已知的年级，饲养在自然可变的温度条件下的参考样品。脂褐素浓度与年龄呈线性相关（r（2）= 92.4%），比传统的基于体型的方法更准确地估计年龄.利用已知年龄的小龙虾模型估算了英国一条河流中野生细鳞螯虾的年龄。脂褐素估计年龄和甲壳长度之间的关系建议在这个野生种群相对缓慢的增长，符合高人口密度和激烈的竞争。该分析还将已知的P，leniusculus寿命延长至约16年。脂褐素法测定年龄和生长可能广泛适用于淡水螯虾，可能进一步的潜力内和外的甲壳类。
1. In ecological studies on freshwater crayfish, determination of basic population parameters is often complicated by the lack of a suitable age estimation method.2. Previously, lipofuscin age pigment in the olfactory lobe cell masses (OLCM) of short-lived tropical crayfish has been used for accurate age determination. Here we present the first test of this method on a longer-lived, temperate species, the signal crayfish, Pacifastacus leniusculus.3. Confocal fluorescence microscopy and image analysis of histological sections were used to quantify OLCM lipofuscin in a reference sample of Swedish P, leniusculus from several known year-classes, reared under naturally variable temperature conditions. Lipofuscin concentration was linearly associated with age (r(2) = 92.4%) and produced much more accurate age estimates than conventional body size-based procedures.4. A model derived from the crayfish of known-age was used to estimate the ages of wild P. leniusculus from an English stream. The relationship between lipofuscin-estimated age and carapace length suggested relatively slow growth in this wild population, consistent with a high population density and severe competition. The analysis also extended the known longevity of P, leniusculus to approximately 16 years.5. The lipofuscin method for determining age and growth may be widely applicable to freshwater crayfish with probable further potential both within and outside the Crustacea.