The BTB/POZ domain protein GmBTB/POZ promotes the ubiquitination and degradation of the soybean AP2/ERF-like transcription factor GmAP2 to regulate the defense response to Phytophthora sojae.
The BTB/POZ domain protein GmBTB/POZ promotes the ubiquitination and degradation of the soybean AP2/ERF-like transcription factor GmAP2 to regulate the defense response to Phytophthora sojae.
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DOI：
10.1093/jxb/erab363
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发表时间：
2021-08
影响因子：
6.9
通讯作者：
Chuanzhong Zhang;Hongying Gao;Yan Sun;Liangyu Jiang;Shengfu He;Bo Song;Shanshan Liu;Ming Zhao-Ming-Z
中科院分区：
文献类型：
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作者：
Chuanzhong Zhang;Hongying Gao;Yan Sun;Liangyu Jiang;Shengfu He;Bo Song;Shanshan Liu;Ming Zhao-Ming-Z
大豆疫霉根茎腐病[甘氨酸max (L.)]稳定。是一种世界性的破坏性疾病。提高大豆对致病病原菌大豆疫霉（Phytophthora sojae）的抗性是育种者的主要目标；然而，目前尚不清楚病原体如何调节宿主体内各种受影响的信号通路，这些信号通路由包括关键转录因子及其靶点在内的复杂网络组成。我们之前已经证明GmBTB/POZ增强了大豆对大豆卟啉菌的抗性和相关的防御反应。在这里，我们报道了GmBTB/POZ与转录因子GmAP2相互作用并促进GmAP2的泛素化。GmAP2-RNAi转基因大豆毛状根对大豆疫病抗性增强，而过表达gmap2的毛状根对大豆疫病过敏。随后，GmWRKY33被鉴定为GmAP2的靶标，GmAP2通过直接结合GmWRKY33启动子抑制GmAP2的表达。GmWRKY33在大豆对大豆病菌的响应中起正向调节作用。此外，GmBTB/POZ的过表达释放了gmap2调控的GmWRKY33在GmAP2-OE大豆毛状根中的表达抑制，增强了其对大豆疫病的抗性。综上所述，这些结果表明GmBTB/POZ-GmAP2调控大豆P. sojae抗性反应是一种新的调控机制，该机制可能调控大豆下游靶基因GmWRKY33。
Phytophthora root and stem rot in soybean [Glycine max (L.) Merr.] is a destructive disease worldwide. Improving soybean resistance to the causal pathogen, Phytophthora sojae, is a major target for breeders; however, it remains largely unclear how the pathogen regulates the various affected signaling pathways in the host, which consist of complex networks including key transcription factors and their targets. We previously demonstrated that GmBTB/POZ enhances soybean resistance to P. sojae and associated defense response. Here, we report that GmBTB/POZ interacts with transcription factor GmAP2 and promotes the ubiquitination of GmAP2. The GmAP2-RNAi transgenic soybean hairy roots exhibited an enhanced resistance to P. sojae, whereas GmAP2-overexpressing hairy roots showed P. sojae hypersensitivity. Subsequently, GmWRKY33 was identified as a target of GmAP2, which represses its expression by directly binding to the GmWRKY33 promoter. GmWRKY33 acts as a positive regulator in the response of soybean to P. sojae. Additionally, the overexpression of GmBTB/POZ released the GmAP2-regulated suppression of GmWRKY33 expression in the GmAP2-OE soybean hairy roots and increased their resistance to P. sojae. Taken together, these results indicate a novel regulatory mechanism, the GmBTB/POZ-GmAP2 modulation of the P. sojae resistance response, which putatively regulates the downstream target gene GmWRKY33 in soybean.