A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study
A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study
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DOI：
10.1016/j.jchromb.2012.02.003
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发表时间：
2012-04-15
影响因子：
3
通讯作者：
Iseki, Ken
中科院分区：
文献类型：
--
作者：
Yamaguchi, Hiroaki;Fujikawa, Asuka;Iseki, Ken
多西他赛是一种紫杉烷类药物，广泛应用于癌症化疗。建立了液相色谱-串联质谱法测定人血浆中多西他赛浓度。血浆样品用含紫杉醇内标物的乙腈脱蛋白。色谱分离在TSKgel ODS-100 V 3 μ m（50 mm × 2.0 mm i.d.）采用乙腈-甲醇-水-甲酸（50：5：45：0.1，v/v/v/v）组成的移动的流动相。采用三重四极杆串联质谱仪，电喷雾离子源，多反应监测（MRM）模式。该方法涵盖的线性范围为5-5000 ng/mL，定量下限为5 ng/mL。日内精密度和日间精密度（R.S.D.）分析的准确率（R.E.）在5、20、200和2000 ng/mL浓度下，在+/- 9.0%范围内。总运行时间为5.0 min。该方法已成功应用于临床药代动力学研究。(C)2012爱思唯尔有限公司版权所有。
Docetaxel is a taxane family antineoplastic agent widely employed in cancer chemotherapy. We developed a liquid chromatography/tandem mass spectrometry method for the determination of docetaxel in human plasma. Plasma samples were deproteinized by acetonitrile containing internal standard paclitaxel. Chromatographic separation was performed on a TSKgel ODS-100V 3 mu m (50 mm x 2.0 mm i.d.) column using a mobile phase composed of acetonitrile-methanol-water-formic acid (50:5:45:0.1, v/v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multi pie reaction monitoring (MRM) mode via electrospray ionization (ESI) source. This method covered a linearity range of 5-5000 ng/mL with the lower limit of quantification of 5 ng/mL. The intra-day precision and inter-day precision (R.S.D.) of analysis were less than 6.7%, and the accuracy (R.E.) was within +/- 9.0% at the concentrations of 5, 20, 200, and 2000 ng/mL. The total run time was 5.0 min. This method was successfully applied for clinical pharmacokinetic investigation. (C) 2012 Elsevier B.V. All rights reserved.