Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
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DOI：
10.1007/s10858-020-00316-y
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发表时间：
2020-04-24
影响因子：
2.7
通讯作者：
Veglia, Gianluigi
中科院分区：
文献类型：
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作者：
Gopinath, T.;Weber, Daniel K.;Veglia, Gianluigi
超快魔角旋转（MAS）技术和H-1检测极大地提高了生物聚合物固态核磁共振（ssNMR）光谱的灵敏度。我们之前的研究表明，当与偏振优化实验（POE）相结合时，这些进步可以使用单个接收器同时获取多维H-1或c -13检测实验。在这里，我们提出了POE家族中的一个新的子类，即HC-DUMAS， HC-MEIOSIS和HC-MAeSTOSO，它们利用双接收器技术同时检测H-1和C-13核。我们还扩展了这种方法，使用三个独立的接收器同时记录H-1， C-13和n -15检测到的同核二维光谱。POE与多接收机技术的结合将进一步缩短生物固体ssNMR实验的总实验时间。
Ultrafast magic angle spinning (MAS) technology and H-1 detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional H-1- or C-13-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of H-1 and C-13 nuclei. We also expand this approach to record H-1-, C-13-, and N-15-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.