A high-temperature water vapor equilibration method to determine non-exchangeable hydrogen isotope ratios of sugar, starch and cellulose.
A high-temperature water vapor equilibration method to determine non-exchangeable hydrogen isotope ratios of sugar, starch and cellulose.
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DOI：
10.1111/pce.14193
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发表时间：
2022-01
影响因子：
7.3
通讯作者：
Lehmann, Marco M.
中科院分区：
文献类型：
--
作者：
Schuler, Philipp;Cormier, Marc-Andre;Werner, Roland A.;Buchmann, Nina;Gessler, Arthur;Vitali, Valentina;Saurer, Matthias;Lehmann, Marco M.
碳水化合物中不可交换氢同位素比（δ 2 Hne）的分析主要限于结构组分纤维素，而非结构碳水化合物（NSC）（如糖和淀粉）的δ 2 Hne值的简单高通量方法尚不存在。在这里，我们测试了最初为纤维素开发的热蒸汽平衡法是否适用于NSC，并通过与传统硝化方法的比较进行了验证。我们建立了一个详细的分析方案，并将该方法应用于来自具有不同光合途径的物种（即，C3、C4和CAM）。来自不同类别和来源的商业糖和淀粉的δ 2 Hne范围为−157.8至+6.4‰，可重复分析，精度在0.2‰至7.7‰之间。糖的平均δ 2 Hne值在C3中最低（−92.0‰），在C4中居中（−32.5‰），在CAM植物中最高（6.0‰），与纤维素相比，NSC是2 H-耗尽的，而糖通常比淀粉更富含2 H-。我们的研究结果表明，我们的方法可以用于未来的研究，以解开2 H分馏过程，改善叶片和树轮纤维素的机械δ 2 Hne模型，并进一步发展植物碳水化合物中的δ 2 Hne作为气候，水文，植物代谢和生理的潜在代理。在这里，我们提出了一种适用于糖和淀粉的高通量δ 2 Hne分析的热水蒸汽平衡方法。此外，我们首次深入了解了在相同恒定气候条件下生长的C3、C4和CAM植物物种内部和之间的δ 2 Hne值差异。
The analysis of the non‐exchangeable hydrogen isotope ratio (δ2Hne) in carbohydrates is mostly limited to the structural component cellulose, while simple high‐throughput methods for δ2Hne values of non‐structural carbohydrates (NSC) such as sugar and starch do not yet exist. Here, we tested if the hot vapor equilibration method originally developed for cellulose is applicable for NSC, verified by comparison with the traditional nitration method. We set up a detailed analytical protocol and applied the method to plant extracts of leaves from species with different photosynthetic pathways (i.e., C3, C4 and CAM). δ2Hne of commercial sugars and starch from different classes and sources, ranging from −157.8 to +6.4‰, were reproducibly analysed with precision between 0.2‰ and 7.7‰. Mean δ2Hne values of sugar are lowest in C3 (−92.0‰), intermediate in C4 (−32.5‰) and highest in CAM plants (6.0‰), with NSC being 2H‐depleted compared to cellulose and sugar being generally more 2H‐enriched than starch. Our results suggest that our method can be used in future studies to disentangle 2H‐fractionation processes, for improving mechanistic δ2Hne models for leaf and tree‐ring cellulose and for further development of δ2Hne in plant carbohydrates as a potential proxy for climate, hydrology, plant metabolism and physiology. Here, we present a hot water‐vapor equilibration method that is suitable for high‐throughput δ2Hne analysis of sugar and starch. Additionally, we show first insights into differences of δ2Hne values within and between C3, C4 and CAM plant species growing under the same constant climatic conditions.
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影响因子：
2.9
作者：
Arosio, Tito;Ziehmer-Wenz, Malin Michelle;Leuenberger, Markus
通讯作者：
Leuenberger, Markus
影响因子：
5.3
作者：
DENIRO, MJ
通讯作者：
DENIRO, MJ
影响因子：
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作者：
Gamarra, Bruno;Kahmen, Ansgar
通讯作者：
Kahmen, Ansgar
影响因子：
2.7
作者：
Cormier, M. -A.;Werner, R. A.;Kahmen, A.
通讯作者：
Kahmen, A.
影响因子：
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作者：
Gaglioti, Benjamin V.;Mann, Daniel H.;Reanier, Richard E.
通讯作者：
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