Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.
Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.
复制标题
用于检测过氧化物酶体-细胞器相互作用的邻近连接测定。
DOI：
10.1007/978-1-0716-3048-8_10
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kamoshita M
中科院分区：
文献类型：
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作者：
Kamoshita M
过氧化物酶体是哺乳动物体内重要的细胞器，参与细胞脂质代谢和氧化还原平衡。它们不作为孤立的实体发挥作用，而是与其他亚细胞器，特别是内质网，线粒体和脂滴合作和相互作用。这些相互作用通常由膜接触位点介导。这些位点的系链蛋白使细胞器紧密靠近，以促进代谢物和脂质转移以及细胞器通信。人们对过氧化物酶体-细胞器接触的生理功能以及它们如何被调节的研究非常感兴趣。在这里，我们提出了一种基于抗体和荧光的邻近连接方法，成功地用于在我们的实验室中培养的哺乳动物细胞中的过氧化物酶体细胞器相互作用的检测和定量。
Peroxisomes are essential organelles in mammals, which contribute to cellular lipid metabolism and redox homeostasis. They do not function as isolated entities but cooperate and interact with other subcellular organelles, in particular the endoplasmic reticulum, mitochondria, and lipid droplets. Those interactions are often mediated by membrane contact sites. Tether proteins at those sites bring the organelles in close proximity to facilitate metabolite and lipid transfer as well as organelle communication. There is great interest in the investigation of the physiological functions of peroxisome-organelle contacts and how they are regulated. Here, we present an antibody-and fluorescence-based proximity ligation approach used successfully in our laboratory for the detection and quantification of peroxisome-organelle interactions in cultured mammalian cells.