The G protein-coupled receptors in the silkworm, Bombyx mori
The G protein-coupled receptors in the silkworm, Bombyx mori
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DOI：
10.1016/j.ibmb.2010.05.005
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发表时间：
2010-08-01
影响因子：
3.8
通讯作者：
Zhou, Naiming
中科院分区：
文献类型：
--
作者：
Fan, Yi;Sun, Peng;Zhou, Naiming
G蛋白偶联受体（GPCR）是细胞中最大且用途最广泛的跨膜受体家族，在许多生理过程的调节中占据最高层次的位置。尽管它们已经在许多模式昆虫中被广泛研究，但是在大型鳞翅目昆虫中，例如家蚕，GPCR的研究很少，家蚕是一种提供进行详细组织表达分析的手段的生物体，这可能有助于表征GPCR及其配体。此外，B.桑（mori）也称为蚕，由于其用于丝绸生产和传统医药，所以是具有重要经济意义的昆虫。在这项工作中，我们计算确定了B中的90个推定的GPCR。mori，其中33个代表新的蛋白质。对这些GPCR进行了注释，并与果蝇和冈比亚按蚊中的同源物进行了比较。对这三种昆虫GPCR的系统发育分析表明，在昆虫进化过程中，GPCR很容易复制或消失，特别是在神经肽和蛋白质激素受体亚家族中。有趣的是，我们观察到在B中，耐胁迫基因Methuselah的数量和多样性都有所下降。这可能与其悠久的驯化历史有关。此外，许多果蝇特异性GPCR的存在表明果蝇和按蚊都不是昆虫纲GPCR的良好代表。(C)2010爱思唯尔有限公司版权所有。
G protein-coupled receptors (GPCRs) are the largest and most versatile family of transmembrane receptors in the cell, occupying the highest hierarchical positions in the regulation of many physiological processes. Although they have been extensively studied in a number of model insects, there have been few investigations of GPCRs in large Lepidopterans, such as Bombyx mori, an organism that provides a means to perform detailed tissue expression analyses, which may help to characterize GPCRs and their ligands. In addition, B. mori, also known as the silkworm, is an insect of substantial economic importance, due to its use in silk production and traditional medicines. In this work, we computationally identified 90 putative GPCRs in B. mori, 33 of which represent novel proteins. These GPCRs were annotated and compared with their homologs in Drosophila melanogaster and Anopheles gambiae. Phylogenetics analyses of the GPCRs from these three insects showed that GPCRs may easily duplicate or disappear during insect evolution, especially in the neuropeptide and protein hormone receptor subfamily. Interestingly, we observed a decrease in the quantity and diversity of the stress-tolerance gene, Methuselah, in B. mori, which may be related to its long history of domestication. Moreover, the presence of many Bombyx-specific GPCRs suggests that neither Drosophila nor Anopheles is good representatives for the GPCRs in the Class Insecta. (C) 2010 Elsevier Ltd. All rights reserved.