MICROTUBULE-ASSOCIATED PROTEIN MAP2 SHARES A MICROTUBULE BINDING MOTIF WITH TAU-PROTEIN
MICROTUBULE-ASSOCIATED PROTEIN MAP2 SHARES A MICROTUBULE BINDING MOTIF WITH TAU-PROTEIN
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DOI：
10.1126/science.3142041
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发表时间：
1988-11-11
期刊：
影响因子：
56.9
通讯作者：
COWAN, NJ
中科院分区：
文献类型：
--
作者：
LEWIS, SA;WANG, DS;COWAN, NJ
The microtubule-associated protein MAP2 is a prominent large-sized component of purified brain microtubules that, like the 36- to 38-kilodalton tau proteins, bears antigenic determinants found in association with the neurofibrillary tangles of Alzheimer''s disease. The complete sequence of mouse brain MAP2 was determined from a series of overlapping clone complementary DNAs. The sequence of the carboxyl-terminal 185 amino acids is very similar (67 percent) to a corresponding region of tau protein, and includes a series of three imperfect repeats, each 18 amino acids long and separated by 13 or 14 amino acids. A subcloned fragment spanning the first two of the 18-amino acid repeats was expressed as a polypeptide by translation in vitro. This polypeptide copurified with microtubules through two successive cycles of polymerization and depolymerization, whereas a control polypeptide derived from the amino-terminal region of MPA2 completely failed to copurifiy. These data imply that the carboxyl-terminal domain containing the 18-amino acid repeats constitutes the microtubule binding site in MAP2. The occurrence of these repeats in tau protein suggests that these may be a general feature of microtubule binding proteins.
