A 39-kDa Capsular Protein is a Major Cross-Protection Factor as Demonstrated by Protection of Chickens with a Live Attenuated Pasteurella multocida Strain of P-1059
A 39-kDa Capsular Protein is a Major Cross-Protection Factor as Demonstrated by Protection of Chickens with a Live Attenuated Pasteurella multocida Strain of P-1059
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DOI：
10.1292/jvms.12-0475
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发表时间：
2013-07-01
影响因子：
1.2
通讯作者：
Sawada, Takuo
中科院分区：
文献类型：
--
作者：
Sthitmatee, Nattawooti;Yano, Terdsak;Sawada, Takuo
本研究的目的是证明多杀性巴氏杆菌菌株P-1059的39-kDa蛋白或OmpH是交叉保护所必需的。以PBA322株为多杀性假单胞菌P-1059的薄胶囊型活疫苗。与亲本菌株P-1059相比，菌株PBA322是一种薄胶囊菌株。鸡单次注射疫苗，然后在接种后两周攻暴露P-1059或X-73菌株。此外，还分别通过ELISA和淋巴细胞增殖试验评估了体液免疫应答和细胞免疫应答。结果表明，活疫苗能有效地保护鸡不受亲本毒株的侵袭，但异源保护作用较差。我们得出结论，39-kDa蛋白对交叉保护至关重要。
The aim of this study was to show that a 39-kDa protein or OmpH of Pasteurella multocida strain P-1059 is essential for cross protection. Strain PBA322, a thinly capsulated strain of P. multocida strain P-1059, was used as a live vaccine in chickens. Strain PBA322 is a thinly capsulated strain in comparison with the parental strain P-1059. Chickens were vaccinated by single injection and then challenge-exposed with strains P-1059 or X-73 at two weeks post vaccination. Moreover, immune responses were also evaluated for both humoral and cellular immune response by ELISA and lymphocyte proliferation assay, respectively. The results showed that the live vaccine induced efficient immunity to protect chickens from challenge-exposure to the parent strain, but that the heterologous protection was poor. We concluded that the 39-kDa protein is essential for cross protection.