Development of hybrid artificial liver support system using spheroid culture and application to warm ischemic liver failure in dog and pig as a preclinical test
Development of hybrid artificial liver support system using spheroid culture and application to warm ischemic liver failure in dog and pig as a preclinical test
复制标题
使用球体培养开发混合人工肝支持系统，并将其应用于狗和猪的热缺血性肝衰竭临床前测试
DOI：
10.1016/s0928-4931(98)00057-5
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
K. Funatsu
中科院分区：
文献类型：
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作者：
M. Kaneko;J. Fukuda;H. Ijima;K. Nakazawa;T. Gion;M. Shimada;K. Shirabe;K. Takenaka;K. Sugimachi;K. Funatsu
我们研制了一种用于大型动物实验的多毛细管聚氨酯泡沫塑料填充床式混合型人工肝组件。在此基础上，我们研制了包含3.0×109个细胞(一个模块)和6.4×109个细胞(两个模块)的体外循环系统，分别应用于热缺血性肝衰竭犬(15 Kg)和猪(25 Kg)。因此，我们的人工肝支持系统很好地维持了狗和猪的血糖浓度，抑制了血氨浓度的上升。应用人工肝支持系统后，猪的存活时间比对照延长约4倍。这些结果表明，MC-PUF填充床组件包括肝细胞球体，是一种很有前途的混合型人工肝支持系统。
We developed a hybrid artificial liver module of multi-capillary polyurethane foam (MC-PUF) packed-bed type for large animal experiments. Primary dog and porcine hepatocytes spontaneously formed spheroids in the module within 24 h. Furthermore, we developed an extracorporeal circulation system, including artificial liver module containing 3.0×109cells (one module) and 6.4×109cells (two modules) which were applied to warm ischemic liver failure dogs (15 kg) and a pig (25 kg), respectively. Consequently, blood glucose concentrations in the dogs and pig were well maintained, increases of blood ammonia concentration were suppressed by use of our artificial liver support system. And survival time of the pig was extended about 4 times more than the control experiments by applying the artificial liver support system. These results indicate that the MC-PUF packed-bed module including hepatocyte spheroids seems to be promising as a hybrid artificial liver support system.