Activation of human pp60(c-src)
Activation of human pp60(c-src)
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DOI：
10.1139/o96-052
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发表时间：
1996-01-01
期刊：
影响因子：
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通讯作者：
Fujita, DJ
中科院分区：
文献类型：
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作者：
Bjorge, JD;OConnor, TJ;Fujita, DJ
pp60c-(src)和src家族的结构相关成员是存在于细胞内与细胞膜相关的非受体酪氨酸激酶，并似乎将信号从跨膜受体传递到细胞内部。Src激活可刺激许多细胞内通路，并可导致多种细胞后果，包括形态改变和细胞增殖。pp60（c-src）的活性通常被一种称为CSK的酶在其羧基末端的磷酸化所抑制。pp60（c-src）内的各种细胞刺激或突变可激活其内源性激酶活性。在本文中，我们回顾了pp60（c-src）激活和调控的各个方面，并讨论了我们实验室在两个实验系统中获得的结果：(i)在黑色素瘤细胞系和原代色素正常的人类黑色素细胞中，以及（ii）使用纯化的人类pp60（c-src）蛋白的激活突变形式。
pp60c-(src) and the structurally related members of the Src family are non-receptor tyrosine kinases that reside within the cell associated with cell membranes and appear to transduce signals from transmembrane receptors to the cell interior. Many intracellular pathways can be stimulated upon Src activation, and a Variety of cellular consequences can result, including morphological changes and cell proliferation. pp60(c-src) activity is normally suppressed by phosphorylation on its carboxyterminal tail by an enzyme known as CSK. Various cellular stimuli or mutations within pp60(c-src) can activate its endogenous kinase activity. In this paper, we review aspects of pp60(c-src) activation and regulation and discuss results obtained in our laboratory in two experimental systems: (i) in melanoma cell lines and primary pigmented normal human melanocytes and (ii) using activated mutant forms of purified human pp60(c-src) protein.