SPINDLE DYNAMICS AND ARRANGEMENT OF MICROTUBULES
SPINDLE DYNAMICS AND ARRANGEMENT OF MICROTUBULES
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DOI：
10.1007/bf00372574
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发表时间：
1973-01-01
期刊：
影响因子：
1.6
通讯作者：
BAJER, A
中科院分区：
文献类型：
--
作者：
JENSEN, C;BAJER, A
研究了加氏血藤胚乳微管排列的变化。在光学显微镜下选择单个细胞，与纺锤体长轴垂直或平行切片。结果表明：在发育后期，动粒纤维(束)总是与非着丝粒(连续)纤维(束)混合在一起。后者经常分叉，并存在一些“自由”末端。通常，一个非动粒纤维与一个以上的动粒纤维相连，这解释了为什么染色体可能失去独立运动的能力。在动粒纤维向极点移动的后期，动粒MTS的数量减少了一半，MTS趋于张开。同时，落后染色体臂之间的MT数目增加，可能代表着动粒MT的片段，这些片段在后期断裂。后期赤道区的非着丝点数量是中期无着丝点数量的两倍。上述数据与偏振光下的数据完全一致，表明在Haemantus的纺锤体中不存在简单的滑动系统。
Changes in microtubule (MT) arrangement were studied in endosperm ofHaemanthus katherinae. Individual cells were selected in the light microscope and sectioned perpendicular or parallel to the long axis of the spindle. The following data and conclusions were drawn: During anaphase kinetochore fibers (bundles of kinetochore MTs) always intermingle with non-kinetochore (continuous) fibers (bundles of non-kinetochore MTs). The latter often branch and some “free” ends are present. Often one non-kinetochore fiber is connected with more than one kinetochore fiber, explaining why chromosomes may lose their ability for independent movement. During anaphase kinetochore fibers move to the poles, the number of kinetochore MTs decreases by one-half and the MTs tend to become more splayed out. At the same time the number of MTs between trailing chromosome arms increases, probably representing segments of kinetochore MTs which break during anaphase. The number of non-kinetochore MTs in the equatorial region at anaphase is twice the number of non-kinetochore MTs in metaphase. The above data agree perfectly with those in polarized light and indicate that a simple sliding system does not exist in the spindle ofHaemanthus.