The physical state of HPV16 infection and its clinical significance in cancer precursor lesion and cervical carcinoma
The physical state of HPV16 infection and its clinical significance in cancer precursor lesion and cervical carcinoma
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HPV16感染的物理状态及其在癌前病变和宫颈癌中的临床意义
DOI：
10.1007/s00432-008-0413-3
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发表时间：
2008-12-01
影响因子：
3.6
通讯作者：
Ma, Ding
中科院分区：
文献类型：
--
作者：
Li, Wei;wang, Wei;Ma, Ding
目的 高危人乳头瘤病毒 (HR-HPV) 整合到宿主 DNA 中已被认为是宫颈癌发生的一个风险。 HPV-16是主要的高危型，其整合率在不同宫颈癌（CC）样本中差异很大。本研究旨在探讨HPV16感染的物理状态与宫颈病变程度的相关性以及病毒存在状态的临床意义。方法对252例癌前病变石蜡包埋块和宫颈癌标本进行HC-II检测HR-HPV感染。同时对HPV16 E7进行PCR和免疫组化检查，证实HPV16感染。通过E2基因3个重叠片段的PCR以及E2基因和E7基因的多重PCR评估HPV16感染的物理状态。结果HR-HPV在正常宫颈组织、宫颈上皮内瘤变(CIN)I、CIN II、CIN III和宫颈癌中的感染率分别为15.0%、32.8%、54.3%、69.7%、93.8%。 HR-HPV阳性样本中有62.8%感染了HPV16。 HPV16在CINⅢ和宫颈癌中的整合率分别为35.7％和58.1％，均显着高于CINⅠ和正常宫颈组织。差异具有统计学意义（P<0.05）。此外，观察到HPV16整合型CIN I比游离型HPV16更常见持续病毒感染和宫颈病变进展。结论HPV16整合率伴随着宫颈病变级别的增加。 HPV16感染的整合状态与持续HPV感染和宫颈病变的进展密切相关。
Purpose Integration of high-risk human papillomavirus (HR-HPVs) into the host DNA has been proposed as a risk for cervical carcinogenesis. HPV-16 is the predominant high-risk type and its integration ration varied largely in different cervical cancer (CC) samples. The aim of this study was to evaluate the correlation between physical state of HPV16 infection and extent of cervical lesion, as well as the clinical significance of virus existing state.Methods A total of 252 cases of paraffin-embedded blocks derived from cancer precursor lesion and cervical carcinoma samples were detected by HC-II for HR-HPV infection. HPV16 infection was confirmed by PCR and immunohistochemistry for HPV16 E7 simultaneously. The physical state of HPV16 infection were assessed by PCR for 3 overlapping fragments in E2 gene and multiple PCR for E2 gene and E7 gene.Results The infection ratio of HR-HPV in normal cervical tissue, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III and cervical cancer were 15.0, 32.8, 54.3, 69.7, 93.8%, respectively. HR-HPV positive samples of 62.8% were infected with HPV16. The integration ratio of HPV16 in CIN III and cervical carcinoma were 35.7 and 58.1% respectively, both of which were significantly higher than that of CIN I and normal cervical tissues. The discrepancy was statistically significant (P < 0.05). Furthermore, it was observed that persistent virus infection and progression of cervical lesion were more common in CIN I with integrated HPV16 than that with episomal HPV16.Conclusion The integration ratio of HPV16 was accompanied by an increase in the grade of cervical lesion. The integrated state of HPV16 infection was strongly associated with persistent HPV infection and progression of cervical lesions.